Abstract: Diferent enulaeationmethods，pretreatment ofrabbit donor embryonic stem cells andthe diferentfieldintensities of electric fusion in the process of nuclear transfer were studied and compared．The results showed that the enueleation rate of ooeytes by the method of extrusion was 85.71％ ．a little better than the method of absorb-infusion．When the electric field intensity Was 1.6 or 1.8 kV／cm，the cleavage rate and the morula／blastocyst rate were higher than other treatments，reaching 33．3％ and 16．67％ ，respectively．Moreover，the rate of mom[a and blastocyst increased when PVA was added to the operation medium．No pregnancy Was observed when 11 reconstructed embryos and 5 reconstructed blastocysts were transferred into the receptor rabbit.

Key words: rabbit(Oryctolagus caniculus), rabbit emb ryonic stem cell, nuclear transfer, embryo clone