Abstract: Seawater solution culturing marine bacterium Loktanella sp．was improved to enhance the production of red pigment．Initially，Plackett-Burman(PB)design WaS used to evaluate the correlation between seawater solution and pi [1ent
production．Among the ten examined factors of the solution，four statistically significant ingredients，which were selected by Plackett—Bunnan design and analyzed by response surface methodology，were utihzed in order to optimize the process in the Box-Behnken design ．The results showed that the production of pigment was double when the components were set as follows：NaCl=0，CaCl2=0．887 g／L，NaHCO3=0．639 g／L，H3BO3=0．058 g／L．

Key words: Loktanclla sp．, saltwater optimization, Box-Behnken design, pigment