Abstract: Principle of pulse electric field （PEF） pasteurization was discussed and the distribution of the PEF in culture fluid was analyzed through simulation， the simulation results showed that pulse widths of the PEF time domain waveform in culture fluid were narrowed at different degrees. Peak of the PEF was higher and penetrating was better when the input pulse rising edge was steeper. Effects of PEF sterilization on Rhizoctorzia solani were studied， liquid of Rhizoctorzia solani diluted to 10-4 were processed in the coaxial treatment chamber with different amplitudes of the electromagnetic pulse （EMP， a frequency of 2.5 Hz） and different action duration. The experiments showed that PEF pasteurization effect could be enhanced by extending the work time when the pulse amplitude was lower; when the amplitude was higher， the main influence factor of PEF pasteurization was the pulse amplitude while it was not very meaningful to extend the work time.

Key words: pulsed electric field, Rhizoctorzia solani, sterilization effect, residual live rate