Abstract: Seven endemic species of Berberis Linn. belonging to the family Berberidaceae， which are Berberis bicolor H. Léveillé， B. guizhouensis T. S. Ying， B. pingbaensis M. T. An， B. tsienii T. S. Ying， B. weiningensis T. S. Ying， B. xanthoclada C. K. Schneider and B. ziyunensis Hsiao & Z. Y. Li， were reported in Guizhou province. B. bicolor H. Léveillé， recorded in China Species Red List， is regarded as rare and endangered species and should be protected as a high priority. B. pruinosa Franchet and B. cavaleriei H. Léveillé were excluded from the checklist of Guizhou endemic plants. The medicinal value and distribution area of the endemic and rare species of Berberis Linn. in Guizhou province were also described. A new checklist of Berberis Linn. in Guizhou province， including 44 species and one variety， was given in this paper; moreover， the problems existing in the genus were analyzed and discussed.

Key words: Berberis Linn., endemic plant, rare and endangered plant