›› 2013, Vol. 25 ›› Issue (4): 0-758.

• 论文 •    

RAPD analysis on somaclonal variation of cabbage (Brasssica oleracea L.var capitata)

LI Gui;TAO Peng;LI Bi-yuan;WANG Wu-hong;YUE Zhi-chen;ZHONG Xin-min;*   

  1. 1Institute of Vegetable Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-07-25 Published:2013-07-25

Abstract: In this experiment, protoplast regenerated plants and no vaccine hypocotyl regenerated plants of cabbage (Brasssica oleracea L.var capitata) were used as materials, using RAPD molecular markers to analyze the somaclonal variation in cabbage regeneration process. The results were as follows: 14 primers which were screened out of 50 primers, using which 253 repeatable loci were amplified including 73 polymorphic loci. This polymorphic loci showed absence of DNA fragments and newly amplified bands. The percentage of polymorphic bands was 2885%. Each primer produced 5.21 polymorphic bands on average. According to RAPD molecular markers, genetic similarity of 17 varieties of cabbage were computed and cluster analysis diagram was drawn using UPGMA. The results showed that: the average genetic similarity was 0.9304, the genetic variation exists in regenerated plants.

Key words: cabbage (Brasssica oleracea L.var capitata), regenerated plants, RAPD, somaclonal variation