Abstract: Usage of the expressional marker\|free vector for plant genetic transformation is a method for obtaining the transgenic plant without selective marker gene. Here， we reported a method for construction of the plant expressional vector based on pCAMBIA1300 backbone by cleavage with the single restriction endonuclease AseⅠ. The constructed vector was used for rice co-transformation， and finally the transgenic rice was obtained without marker gene， which indicated that the potential vector application was to obtain the marker-free plant by co-transformation.

Key words: marker-free, expressional vector, transgene, biological safety