Expression， purification and polyclonal antibody preparation of recombinant Lv-PEN-3 protein

›› 2013, Vol. 25 ›› Issue (5): 0-940.

• 论文 •

Expression， purification and polyclonal antibody preparation of recombinant Lv-PEN-3 protein

DU Zhi-qiang

School of Mathematics， Physics and Biological Engineering， Inner Mongolia University of Science and Technology， Baotou 014010, China

Received:1900-01-01 Revised:1900-01-01 Online:2013-09-25 Published:2013-09-25

Abstract

Abstract: In this paper， Litopenaeus vannamei penaeidin 3 was used as the research object. The construction of E. coli recombinant expression vectors， induced expression and purification of recombinant protein， and polyclonal antibody preparation were investigated respectively. The results showed that Lv-PEN-3 could be correctly expressed in E. coli expression system. And this recombinant protein possessed good immunity stimulation function. It could stimulate rabbit to produce corresponding antibody.

Key words: Litopenaeus vannamei, penaeidin, induced expression, purification, polyclonal antibody preparation

DU Zhi-qiang. Expression， purification and polyclonal antibody preparation of recombinant Lv-PEN-3 protein[J]. , 2013, 25(5): 0-940.

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Expression， purification and polyclonal antibody preparation of recombinant Lv-PEN-3 protein

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