Abstract: Ciguatera fish poisoning （CFP） was a global food-borne illness caused by consumption of sea food containing ciguatoxin （CTX）. P-CTX-1 had been suggested to be the most toxic CTX， which caused ciguatera at 01μg in the flesh of carnivorous fish. A sensitive and rapid method for the detection of ciguatoxin in coral fish using ASE-SPE-HPLC/MS/MS was developed in the study. The calibration curve of P-CTX1 showed good linearity in the range of 1.0-50.0 μg·kg-1 with correlation coefficient of 0.99. The validated method had limits of detection （LOD） and quantification （LOQ） of 0.03 μg·kg-1 and 0.1μg·kg-1， respectively， for matrix-based standard solutions， as well as matrix spike recoveries ratio above 95%. Compared with previous methods， this method had better overall recovery and extraction efficiency.

Key words: ciguatoxin, accelerated solvent extraction, solid phase extraction, HPLC-MS/MS