Abstract: A comparative analysis with clenbuterol chemiluminescence enzyme\|linked immunoassay（CLEIA） kit and clenbuterol enzyme\|linked immunosorbent assay（ELISA） kit was used to determined the clenbuterol contents in samples of porcine urine and pork. The results showed that the testing time of the CLEIA kit was 33 min， and that of the ELISA kit was about 50 min， so the CLEIA kit cost less time than ELISA kit. The IC50 of the CLEIA kit was 400 ng·L-1， and the limit of detection for urine and pork were 201 ng·L-1 and 907 ng·kg-1， respectively， and the homologous parameters of ELISA kit were 1252 ng·L-1， 235 ng·L-1and 993 ng·kg-1， so the sensibility of CLEIA method was better than ELISA method. In summary， the CLEIA method was more sensitive and cost less time than ELISA method.

Key words: clenbuterol, chemiluminescence enzyme\|linked immunoassay, enzyme\|linked immunosorbent assay