Abstract: Strawberry mottle virus（SMoV） and Strawberry mild yellow edge virus（SMYEV）， which are found world\|wide， can cause serious crop losses. Total RNA for reverse transcriptase\|polymerase chain reaction （RT\|PCR） was extracted from strawberry with CTAB method. A multiplex RT\|PCR assay was used for simultaneous detecting SMoV and SMYEV based on ndh B gene as the internal control. Both field plants and test\|tube plantlets of strawberries were detected effectively.

Key words: strawberry virus, internal control, multiplex RT\|PCR, virus detection