Abstract: Lily symptomless virus （LSV） is one of the main viruses infecting Liliaceae plants. In the study， a real\|time fluorescent RT\|PCR method for LSV detection was established with specific primers and probe designed from the conservative sequences in coat protein genes of LSV isolates. Specificity study showed that typical amplification curves could be obtained for two LSV\|positive samples， but not for Lily mottle virus， Tobacco ringspot virus and Tomato ringspot virus\|positive samples. Sensitivity of this method was 10 times higher than that of regular RT\|PCR. With this method， 53 batches of LSV were intercepted in 67 batches of lily imported from Holland since June 2012 by Ningbo Entry\|Exit Inspection and Quarantine Bureau， and the detection rate was 93%.

Key words: Lily symptomless virus （LSV）, real\|time fluorescent RT\|PCR, Liliaceae