Abstract: An 8week feeding trial was conducted to investigate the effects of dietary biotin on intestinal digestible enzymes activities， carcass composition and hepatic antioxidant capability for juvenile blunt snout bream (Megalobrama amblycephala). Quadruple groups of fish (initial average weight 201±001g) were fed thrice daily with six isonitrogenous and isoenergetic purified diets containing 0 (basal diet)， 002， 005， 016， 062 and 249 mg·kg-1 biotin， respectively. Results showed that intestinal lipase and protease activities increased initially and then decreased slightly with the dietary biotin levels increased from 0 to 249 mg·kg-1， the maxima reached at 062 and 016 mg·kg-1， respectively. Fish fed with the diet with biotin 016 mg·kg-1 had lower carcass moisture content and higher carcass crude lipid and crude protein contents than those fed with the basal diets， the differences were significant (P<005). GSH， GSHPx and SOD increased significantly (P<005) as dietary biotin levels increased from 0 to 005 mg·kg-1 and then had plateaued. Results indicated that appropriate dietary biotin can improve intestinal lipase and protease activities， carcass lipid and crude protein content and hepatic antioxidant capacity of juvenile blunt snout bream．

Key words: blunt snout bream, biotin, intestinal digestible enzyme, carcass composition, hepatic antioxidant capability