Abstract: A plant regeneration system from leaves and stems of tissuecultured Ajania purpurea was developed in this study. The results showed that the callus was induced with a rate of 956% on an optimal medium MS+10 mg·L-1 6BA+01 mg·L-1 NAA. The highest induction rate of the adventitious shoots from the calli on the medium of MS+10 mg·L-1 6BA+02 mg·L-1 NAA was 82%. The optimal medium for adventitious root inducing was 1/2MS+015 mg·L-1 IBA， with a rooting rate of 90%. The plantlets were transplanted to pots with sands， vermiculite and peat to acclimate for 2-3 weeks. Then the survival plantlets were transferred outdoors with 90% transplanting survival rate．

Key words: Ajania purpurea, explants from stems and leaves, regeneration system