›› 2014, Vol. 26 ›› Issue (2): 0-365370.

• 植物保护 •    

Rice black streaked dwarf virus P91,selfinteracts and forms inclusion bodies in epidermal cells of Nicotiana benthamiana plants

TAN Zilong;SUN Liying;*;CHEN Jianping;*   

  1. 1College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;2State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection/Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-03-25 Published:2014-07-09

Abstract: Rice blackstreaked dwarf virus (RBSDV), a member of the genus Fijivirus, is transmitted in nature by the small brown planthopper (Laodelphax striatellus) and can replicate in cells of both host plants and insect vector. RBSDV, like other members of the family Reovirus, is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms. It has genomic segments of 10 dsRNAs (S1-S10) and P91 encoded by the first open reading frame in the ninth segment (S9) of the genomic RNAs is an essential protein for the formation of viroplasms. Fused with green fluorescent protein (GFP), P91 was able to form inclusion bodies when expressed alone in Nicotiana benthamiana epidermal cell. Bimolecular fluorescence complementation (BiFC) assay suggested P91 had the ability of selfinteraction and formed inclusion bodies.

Key words: Rice black streaked dwarf virus, inclusion bodies, selfinteraction