Abstract: The purity and integrity of total RNA are essential for molecular biology experiments of Auricularia auricular. In order to obtain an ideal total RNA extraction method， total RNA of Auricularia auricular was extracted with 3 methods， including Trizol method， CTAB method and RNA prep pure Plant Kit method. The results showed that all these methods can extract total RNA from Auricularia auricular; however， the Trizol method was the best with high integrity and purity. The band of 28S was twice wider than that of 18S. The RNA yield was 68264 μg·g-1 and the RNA sample could be used for RTPCR.

Key words: Auricularia auricular, RNA extraction, Trizol method, RTPCR