Abstract: Pleurotus abalonus has a high nutritional value， with a very special and delicious taste. But its output is very low. There are some limitations and subjectivities to its classification and determination in terms of traditional morphological features， so that the collected wild P. abalonus should be further identified for its reality. In the study， the rDNA ITS sequences of the fruiting bodies and their isolates of wild P. abalonus were amplified via PCR using ITS1 and ITS4 as primer pairs. And their sequences were sequenced by clone sequencing. Molecular identification was carried out by GenBank search and ITS phylogenetic tree analysis. Combined with analysis of morphological features， the results showed that the collected wild mushroom was P. abalones. The work indicated important value of ITS sequence in identification of the different strains of mushroom.

Key words: clone sequencing, isolate, ITS sequence