Isolation, identification and biological characteristics of a wild Flammulina filiformis strain

doi:10.3969/j.issn.1004-1524.2023.05.12

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (5): 1088-1096.DOI: 10.3969/j.issn.1004-1524.2023.05.12

• Horticultural Science • Previous Articles Next Articles

Isolation, identification and biological characteristics of a wild Flammulina filiformis strain

FENG Lianrong¹(), ZHANG Yan¹, ZHAO Xinwen¹, SONG Lizhi¹, LIANG Dejun²^,^*()

1. Liaoning Provincial Institute of Poplar, Gaizhou 115213, Liaoning, China
2. Liaoning Institute of Economic Forestry, Dalian 116030, Liaoning, China

Received:2022-06-17 Online:2023-05-25 Published:2023-06-01

Abstract

Abstract:

The isolation, identification and biological characteristics of a wild edible fungus were studied to provide candidate strain resources for the development and utilization of wild edible fungi. The wild macrofungi JC423 collected from poplar stumps was taken as the research object, and its taxonomic status was clarified through morphological identification, fruiting test, ITS sequences analysis and phylogenetic analysis. The effects of different carbon sources, nitrogen sources, inorganic salts, vitamins, pH and temperature on the growth of JC423 hyphae were studied by single factor experiment. The results showed that the collected wild edible fungi were preliminarily identified as Flammulina velutiper according to the shape of the fruiting bodies. The 773 bp target fragment was obtained by ITS sequence analysis. BLAST sequence alignment showed that the highest similarity between this strain and F. filiformis strain F007 was 99.34%. Partially similar sequences were downloaded and an evolutionary tree was constructed by MEGA 7.0. JC423 and F. filiformis strain F007 with the serial number MN337400.1 were clustered together. Combined with morphological characteristics, JC423 was identified as F. filiformis. The results of biological characteristics showed that the suitable carbon source for the growth of JC423 mycelium was fructose, the suitable nitrogen source was peptone, the suitable pH range was 9-10, and the suitable growth temperature was 25 ℃. Inorganic salts and vitamins had little effect on mycelial growth. The study clarified the nutritional requirements and conditions for the growth of wild F. filiformis, and laid a foundation for the development and utilization of F. filiformis.

Key words: Flammulina filiformis, strain isolation, ITS sequence, biological characteristic

CLC Number:

S646.15

FENG Lianrong, ZHANG Yan, ZHAO Xinwen, SONG Lizhi, LIANG Dejun. Isolation, identification and biological characteristics of a wild Flammulina filiformis strain[J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1088-1096.

Figures/Tables 7

References 34

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配方 Formula	菌丝长满袋时间 Time takes for hyphae to fill the bag/d	采收期 Harvest period/d	菇体整齐度 Mushroom uniformity	平均单袋产量 Average bag yield/g	袋数 Number of bags	总产量 Total yield/g	生物学效率 Biological efficiency/%
1	38	70	整齐Neat	330.15 aA	42	13 688.5	65.18
2	40	70	较整齐Relatively neat	298.00 aAB	42	12 516.0	59.60
3	40	68	整Neat	148.44 cC	42	6 232.48	29.68
4	41	71	较整齐Relatively neat	230.84 bB	42	9 695.28	46.17

配方 Formula	菌丝长满袋时间 Time takes for hyphae to fill the bag/d	采收期 Harvest period/d	菇体整齐度 Mushroom uniformity	平均单袋产量 Average bag yield/g	袋数 Number of bags	总产量 Total yield/g	生物学效率 Biological efficiency/%
1	38	70	整齐Neat	330.15 aA	42	13 688.5	65.18
2	40	70	较整齐Relatively neat	298.00 aAB	42	12 516.0	59.60
3	40	68	整Neat	148.44 cC	42	6 232.48	29.68
4	41	71	较整齐Relatively neat	230.84 bB	42	9 695.28	46.17

处理 Treatment		菌丝长势 Hyphae growth	菌丝密度 Hyphael density	生长速度 Speed of growth
碳源Carbon source	果糖Fructose	+++	浓密Dense mycelium	11.52±1.14 aA
	甘露醇 Mannitol	++	稀疏Sparse	9.41±1.30 bAB
	可溶性淀粉 Soluble starch	+++	浓密Dense mycelium	7.95±1.66 bcBC
	蔗糖 Sucrose	++	较密Relatively dense	7.10±1.30 cdBC
	葡萄糖 Glucose	++	较密Relatively dense	6.72±1.75 cdBC
	对照CK	+	稀疏Sparse	5.25±0.33 dC
氮源Nitrogen source	蛋白胨 Peptone	+++	浓密Dense mycelium	12.56±0.31 aA
	酵母膏Ysast extract	++	浓密Dense mycelium	12.44±0.14 aA
	硫酸铵Ammonium sumlphate	++	较密Relatively dense	10.91±1.16 bAB
	硝酸铵Ammonium nitrate	++	较密Relatively dense	10.35±1.41 bcB
	尿素Urea	+++	浓密Dense mycelium	9.00±0.84 cB
	对照CK	+	稀疏Sparse	9.87±1.12 bcB
无机盐Inorganic salt	氯化钠 Sodium chloride	+++	浓密Dense mycelium	12.45±0.26 aA
	硫酸钙 Calcium sulfate	+++	浓密Dense mycelium	12.43±0.85 aA
	硫酸镁 Magnesium sulfate	+++	浓密Dense mycelium	12.21±0.21 aA
	磷酸二氢钾Potassium dihydrogen phosphate	+++	较密Relatively dense	11.54±0.57 aA
	对照CK	+++	较密Relatively dense	11.75±0.58 aA
维生素Vitamin	V_B3	+++	浓密Dense mycelium	8.17±1.22 aA
	V_B1	+++	浓密Dense mycelium	8.06±0.66 aA
	V_B12	+++	浓密Dense mycelium	8.02±0.72 aA
	V_B2	++	浓密Dense mycelium	7.64±1.22 aA
	V_B6	++	较密Relatively dense	6.95±1.22 aA
	对照CK	++	较密Relatively dense	7.43±1.38 aA
pH	4	+++	浓密Dense mycelium	8.43±0.86 bcBCD
	5	+++	浓密Dense mycelium	8.96±0.48 abABC
	6	++	较密Relatively dense	8.00±0.72 cdCD
	7	++	较密Relatively dense	7.45±0.38 dDE
	8	++	较密Relatively dense	6.59±0.34 eE
	9	+++	浓密Dense mycelium	9.34±0.50 aAB
	10	+++	浓密Dense mycelium	9.64±0.22 aA
温度Temperature/℃	5	++	稀疏Sparse	2.32±0.21 eE
	10	++	较密Relatively dense	5.66±0.77 cC
	15	++	较密Relatively dense	4.39±0.21 dD
	20	+++	较密Relatively dense	9.70±0.44 bB
	25	+++	浓密Dense mycelium	11.37±0.09 aA
	30	+++	较密Relatively dense	9.70±0.60 bB
	35	-	-	0 fF

处理 Treatment		菌丝长势 Hyphae growth	菌丝密度 Hyphael density	生长速度 Speed of growth
碳源Carbon source	果糖Fructose	+++	浓密Dense mycelium	11.52±1.14 aA
	甘露醇 Mannitol	++	稀疏Sparse	9.41±1.30 bAB
	可溶性淀粉 Soluble starch	+++	浓密Dense mycelium	7.95±1.66 bcBC
	蔗糖 Sucrose	++	较密Relatively dense	7.10±1.30 cdBC
	葡萄糖 Glucose	++	较密Relatively dense	6.72±1.75 cdBC
	对照CK	+	稀疏Sparse	5.25±0.33 dC
氮源Nitrogen source	蛋白胨 Peptone	+++	浓密Dense mycelium	12.56±0.31 aA
	酵母膏Ysast extract	++	浓密Dense mycelium	12.44±0.14 aA
	硫酸铵Ammonium sumlphate	++	较密Relatively dense	10.91±1.16 bAB
	硝酸铵Ammonium nitrate	++	较密Relatively dense	10.35±1.41 bcB
	尿素Urea	+++	浓密Dense mycelium	9.00±0.84 cB
	对照CK	+	稀疏Sparse	9.87±1.12 bcB
无机盐Inorganic salt	氯化钠 Sodium chloride	+++	浓密Dense mycelium	12.45±0.26 aA
	硫酸钙 Calcium sulfate	+++	浓密Dense mycelium	12.43±0.85 aA
	硫酸镁 Magnesium sulfate	+++	浓密Dense mycelium	12.21±0.21 aA
	磷酸二氢钾Potassium dihydrogen phosphate	+++	较密Relatively dense	11.54±0.57 aA
	对照CK	+++	较密Relatively dense	11.75±0.58 aA
维生素Vitamin	V_B3	+++	浓密Dense mycelium	8.17±1.22 aA
	V_B1	+++	浓密Dense mycelium	8.06±0.66 aA
	V_B12	+++	浓密Dense mycelium	8.02±0.72 aA
	V_B2	++	浓密Dense mycelium	7.64±1.22 aA
	V_B6	++	较密Relatively dense	6.95±1.22 aA
	对照CK	++	较密Relatively dense	7.43±1.38 aA
pH	4	+++	浓密Dense mycelium	8.43±0.86 bcBCD
	5	+++	浓密Dense mycelium	8.96±0.48 abABC
	6	++	较密Relatively dense	8.00±0.72 cdCD
	7	++	较密Relatively dense	7.45±0.38 dDE
	8	++	较密Relatively dense	6.59±0.34 eE
	9	+++	浓密Dense mycelium	9.34±0.50 aAB
	10	+++	浓密Dense mycelium	9.64±0.22 aA
温度Temperature/℃	5	++	稀疏Sparse	2.32±0.21 eE
	10	++	较密Relatively dense	5.66±0.77 cC
	15	++	较密Relatively dense	4.39±0.21 dD
	20	+++	较密Relatively dense	9.70±0.44 bB
	25	+++	浓密Dense mycelium	11.37±0.09 aA
	30	+++	较密Relatively dense	9.70±0.60 bB
	35	-	-	0 fF

Isolation, identification and biological characteristics of a wild Flammulina filiformis strain

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[2]	WANG Zhipeng, ZHAO Jian, HUANG Pan, CUI Xuemei, NAN Li, SONG Houhui, BAO Guolian, LIU Yan. Isolation, identification and biological characteristics of rabbit-derived Escherichia coli bacteriophage [J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1599-1608.
[3]	YANG Ling, SHA Nanjing, PAN Pengju, WU Bozhi. Identification and main biological characteristics of pathogen of Clematis leaf blight in Yunnan [J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1449-1456.
[4]	XU Jianjun, MA Yan, WU Qichao, WANG Baosheng, ZANG Dekui. Screening and analysis of genome sequence difference in wild rose [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1032-1038.
[5]	CHEN Runchen, WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei. Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa [J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2330-2338.
[6]	LI Xuqing, YAN Jianli, RUAN Songlin. Identification and biological characteristics of anthracnose pathogen on Tetrastigma hemsleyanum [J]. , 2020, 32(11): 2009-2019.
[7]	LI Xuqing, ZHANG Jingze, ZHANG Ya, WU Genliang. Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province [J]. , 2019, 31(5): 784-789.
[8]	ZHOU Huiming, ZHANG Yanzhen, CHAI Hongmei, YANG Rongqing, TAN Ying, ZHANG Pingping, BAI Yuying, ZHAO Yilian, JIN Yujie. Identification and culture conditions of a wild-type Termitomyces aurantiacus strain from Lincang [J]. , 2019, 31(10): 1655-1662.
[9]	MA Xiaoping, YANG Qiuxia, YU Yan, LI Desheng, WANG Chengdong, LING Shanshan, GU Yu. Comparison of partial biological characteristics and drug sensitivity between Cladosporium cladosporioides wild strain (Z20) from giant panda and mutant strain (Zt) [J]. , 2018, 30(8): 1328-1335.
[10]	LEI Xueping, GENG Yi, YU Zehui, ZHENG Liping, CAO Shiqi, HUANG Xiaoli, CHEN Defang, OUYANG Ping, LIU Kairui. Isolation and identification of Elizabethkingia meningoseptica from Chinese spiny frog (Quasipaa spinosa) and pathological lesions of its infection [J]. , 2018, 30(3): 371-377.
[11]	MA Xiaoping, YANG Tianyi, ZHANG Zhihe, YU Yan, WANG Chengdong, GU Yu. Study on biological characteristics of Lactobacillus from giant panda vagina [J]. , 2017, 29(7): 1093-1102.
[12]	ZOU Li， YANG Yuan\\|yi， SUN Ting\\|ting， WANG Shi\\|xin， CUI Rong， LI Jing\\|ying. Isolation and identification of wild Lepista sordida by ITS gene sequence [J]. , 2016, 28(2): 264-.
[13]	LI Jing\\|jing， DING Song\\|shuang， LI Yan\\|ping， YUN Fei， YAN Hai\\|tao， WANG Zhi\\|meng， LIU Guo\\|shun*. Effects of biochar and nitrogen fertilizers on dry matter accumulation of flue\\|cured tobacco and soil biological characteristics [J]. , 2016, 28(1): 96-.
[14]	WANG Qiao\\|ying， ZHANG Xue， ZUO Shi\\|xuan， ZHENG Meng\\|ru， ZHANG Meng\\|qiong， ZHANG Yan\\|ru， JIANG Ming*. Cloning and analysis of rDNA ITS sequences from Vanda plants#br# [J]. , 2015, 27(8): 1362-.
[15]	ZHOU Xiao\\|jun1,*， HE Xiao\\|chan1， ZHENG Zhai\\|sheng1， ZHANG Shang\\|fa1， SUN Yu\\|jian1， LOU Bing\\|gan2. Study on the biological characteristics of Fusarium oxysporum Schl. f. sp. Nelumbicola （Nis. & Wat.） Booth [J]. , 2015, 27(2): 211-.