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RAPD analysis of genetic relationship of 9 varieties of Arundo donax L.

  

  1. (1 Fujian Agricultural and Forestry University, Fuzhou 350000, China;2 China National Engineering Research Center of Juncao Technology, Fuzhou 350000, China)
  • Online:2015-04-25 Published:2015-04-30

Abstract: In this study, genetic diversity and relationships of 9 different Arundo donax L. germplasms were analyzed using random amplified polymorphic DNA (RAPD) markers in order to provide essential information for hybridization. RAPD analysis demonstrated that 13 random primers produced 126 loci among which 113 were polymorphic loci, the percentage of polymorphic loci was 897%, and each primer produced 7-14 bands. Genetic distance changed from 0 to 1039 1, with the average value of 0399 6, and the genetic similarity was among the range of 0309 5 to 1000 0, with the average value of 0734 3. According to clustering analysis diagram drawn using UPGMA, the 9 materials were classified into three groups by 07: lyuzhouNo.5 and lyuzhouNo.6 was separated from other materials. The cluster results showed that genetic distance was not related to geographic distance, which cant reflect a certain geographic distribution.

Key words: Arundo donax L.;genetic diversity, RAPD analysis