Abstract: A pot experiment was conducted to provide theoretical and practical basis for the large scale application of different ratios of coconut coir and red soil on growth of Brazil banana plantlets. It was shown that application of certain proportion of coconut coir into red soil could significantly improve physical and chemical properties of culture medium and improve the growth of banana plantlets. Application of high proportion of coconut coir into red soil could significantly improve the photosynthetic efficiency， plant height， root length， root surface area， root projection area， root volume， total root tip， branching and crossing numbers. After comprehensive evaluation of agronomic traits and photosynthetic efficiency among 4 culture media， 1∶1 （coconut coir: red soil） volume ratio was found the best for Brazil banana plantlets growth.

Key words: coconut coir, tissue culture seedling, agronomic traits, photosynthetic efficiency