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Identification of Aphelenchus sp. in continuouscropping strawberry rhizosphere soil from Beijing

  

  1. (1. Key Laboratory of Integrated Pest Management on Crops in Southwest, Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 2. Laboratory of Entomology and Nematology, College of Plant Protection, China Agricultural University, Beijing 100193, China; 3. National Engineering Laboratory for Grain Storage and Logistics, School of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China; 4. Shandong Provincial Key Laboratory of Dryland Technology, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China)
  • Online:2016-06-25 Published:2016-06-12

Abstract: Aphelenchus sp.,a dominant nematode species in Beijing continuouscropping strawberry soil, was identified in present study by morphological and molecular methods. The results showed that its morphology was extremely similar with Aphelenchus avenae Bastian. Besides, the main morphological measurements of Aphelenchus sp. and Aphelenchus avenae Bastian were consistent. On the basis of morphological identification results, combining with ITS rDNA regional sequence amplification results with GenBank registered sequence alignment analysis, it was determined that Aphelenchus sp. was a new strain of Aphelenchus avenae. The identification of Aphelenchus sp. laid a solid foundation for the further study of its biology and ecology characteristics. Since Aphelenchus sp. existed in large numbers in the rhizosphere soil of continuouscropping strawberry, its needed to carry out further research to know whether it have adverse effect on the growth of strawberry or not.

Key words: continuouscropping strawberry, taxonomic identification, fungivorous nematode, Aphelenchus avenae Bastian