Abstract: In order to realize the rapid propagation of Pueraria lobata（wild） Ohwi Dabashan， the effects of sterilization time， macroelement in MS medium and plant growth regulators on disinfection， initial culture， multiplication culture and rooting culture of Pueraria lobata（wild） Ohwi Dabashan were studied by tissues culture， using its stem with axillary bud as explants. The results showed that: （1） The explants were sterilized in 75% alcohol and 0.1% HgCl2 for 20 s and 10 min， the survival rate reached to 67.56%. （2） The medium for the initial culture was 1/2 MS+0.5 mg·L-1 6-BA+0.3 mg·L-1 IBA， the percentage of axillary bud was up to 92.2%. （3） The optimum multiplication medium was 1/2 MS+1.0 mg·L-1 6-BA+0.05 mg·L-1 IBA， multiplication coefficient reached to 5.0. （4） The rooting medium was 1/2 MS+0.01 mg·L-1 NAA+0.1 mg·L-1 IBA， with rooting rate 96.03%.

Key words: Pueraria lobata（wild） Ohwi Dabashan, tissues culture, axillary bud sprouting, plant growth regulator