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Effect of soybean isoflavone on NGF, IL-2 protein in ovariectomized rats spleen

  

  1. (1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2. Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 3. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China)
  • Online:2016-07-25 Published:2016-07-08

Abstract: To study the effect of soybean isoflavone on NGF, IL-2 protein in the ovariectomized rats spleen, young female SD rats were ovariectomized successfully, and then were supplied with soy isoflavone (SI) in high dosage (1.5 mg·kg-1), medium dosage (1.0 mg·kg-1), low dosage (0.5 mg·kg-1), respectively. The rats of sham operation group and the control group were supplied with solvent agent. All of the above groups endured hypodermic injection once every other day. After 14 and 42 d treatment, 5 rats per group were killed for study. The expression and distribution of NGF, IL-2 in spleen were studied by immunohistochemical methods. The NGF, IL-2 protein were mainly distributed in red pulp close to the capsule. The intensity and number of NGF, IL-2 positive cells of the ovariectomized rats were significantly decreased, and after ovariectomizing, the damage was enhanced with prolonged time. After soy isoflavone treatment, the intensity and number of NGF and IL2 positive cells of ovariectomized rats increased and showed a timedependent manner. During the same period, with the increased concentration of soy isoflavone, the intensity and number of NGF and IL-2 positive cells increased and showed a dosedependent manner. The above results suggested that soy isoflavone could upregulate the expression of NGF and IL2 in a timeand dosedependent manner.

Key words: soy isoflavone, immunohistochemical, NGF, IL-2, spleen