Expression and distribution of OB-Rb and NPY in ovary of giant panda

doi:10.3969/j.issn.1004-1524.2016.12.06

›› 2016, Vol. 28 ›› Issue (12): 2000-2006.DOI: 10.3969/j.issn.1004-1524.2016.12.06

• Animal Science • Previous Articles Next Articles

Expression and distribution of OB-Rb and NPY in ovary of giant panda

LUO Qi-hui¹, WANG Cheng-dong², LI De-sheng², DENG Lin-hua², LI Cai-wu², CHEN Zheng-li^1,*, HUANG Chao¹

1. Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province, China Conservation and Research Center for the Giant Panda, Ya'an 625014, China;

Received:2016-04-29 Online:2016-12-15 Published:2017-01-05

Abstract

Abstract: In order to explore the effect of long-form leptin receptor (OB-Rb) and neuropeptide Y (NPY) on reproductive regulation of giant panda, the ovarian histology structure, expression of OB-Rb and NPY in ovaries of 5 cases of giant panda were studied by HE staining and immunohistochemical SABC. Results showed that the numbers of ovarian follicles in different stages were small, especially the middle or late follicle, while the numbers of primitive follicles and atresia follicles were relatively greater. OB-Rb and NPY were expressed in ovaries. The OB-Rb positive products were distributed in granulosa cells, theca cells and granular lutein cells of corpus luteum. The NPY positive fiber and products were unevenly distributed in ovarian parts, which formed as beads or dots. The NPY positive nerve mainly distributed around blood vessels, in follicular, corpus luteum and granulosa cells. Results showed that small number of follicle was the features of giant panda's ovarian, which may be closely related with its low reproductive ability, and OB-Rb and NPY participated in the regulation of ovarian follicle development.

Key words: NPY, OB-Rb, immunohistochemical, ovary, giant panda

CLC Number:

S864.1

LUO Qi-hui, WANG Cheng-dong, LI De-sheng, DENG Lin-hua, LI Cai-wu, CHEN Zheng-li, HUANG Chao. Expression and distribution of OB-Rb and NPY in ovary of giant panda[J]. , 2016, 28(12): 2000-2006.

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Expression and distribution of OB-Rb and NPY in ovary of giant panda

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