›› 2018, Vol. 30 ›› Issue (7): 1132-1136.DOI: 10.3969/j.issn.1004-1524.2018.07.05

• Animal Science • Previous Articles     Next Articles

Molecular characterization of Enterocytozoon bieneusi in captive giant panda, China

CAO Yuying1, LI Wei1, ZHONG Zhijun1, XIAO Jie1, GONG Chao1, WANG Chengdong2, LI Caiwu2, YANG Haidi2, HUANG Xiangming3, WU Kongju3, *, PENG Guangneng1, *   

  1. 1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. Wolong Base of China Conservation and Research Center for Giant Panda, Aba 624000, China;
    3. Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China
  • Received:2017-11-02 Online:2018-07-20 Published:2018-08-02

Abstract: In order to reveal the prevalence of Enterocytozoon bieneusi in captive giant panda and assess its zoonotic potential, we surveyed 31 fecal samples from captive giant panda based on nested PCR amplification of ITS gene of E. bieneusi. A total of 14 samples were positive for E. bieneusi, with an infection rate of 45.2%. Two genotypes were identified as SC02(13/14)and Peru6(1/14). Phylogenetic analysis revealed that these genotypes belonged to group 1b which had zoonotic potential. Giant panda could spread E. bieneusi to humans and lead to human microsporidiosis. The first report of SC02 in giant panda broadened the understanding of E. bieneusi of hosts.

Key words: Enterocytozoon bieneusi, giant panda, nested PCR, ITS gene, zoo

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