Epidemiological survey of porcine sapelovirus and genetic variation analysis of 1B gene in Sichuan Province

doi:10.3969/j.issn.1004-1524.2016.12.05

›› 2016, Vol. 28 ›› Issue (12): 1992-1999.DOI: 10.3969/j.issn.1004-1524.2016.12.05

• Animal Science • Previous Articles Next Articles

Epidemiological survey of porcine sapelovirus and genetic variation analysis of 1B gene in Sichuan Province

LI Yu-meng^a, GUO Bo^a, XU Yi-Fei^a, CHEN Ying-qi^a, CAI Yao^a, LI Xiao-Jing^a, GONG Shuang-yan^a, XU Zhi-wen^a,b, ZHU Ling^a,b,*

a. Animal Biotechnology Center; b. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-05-13 Online:2016-12-15 Published:2017-01-05

Abstract

Abstract: To investigate the prevalence of porcine sapelovirus (PSV), a total of 428 fecal samples from pigs with diarrhea were collected from 34 pig farms during 2015 to 2016 in Sichuan Province, and PSV in these samples was detected by using qRT-PCR. The results revealed that 114 (26.6%) samples and 20 (58.8%) farms were positive for PSV, which indicated that PSV infections in pig herds were endemic in Sichuan province. The 14 novel PSV 1B nucleotide sequences were obtained by RT-PCR and confirmed by sequencing (GenBank accession No.: KU323644-KU323657). Nucleotide and the deduced amino acid sequence homologies were 90.6%-100% and 97.1%-100%, respectively. Phylogenetic analysis indicated that the novel Sichuan strains belong to a group with the strains from other provinces of China. There was no major gene mutation, which showed that the gene of PSV was highly conserved in China. The novel Sichuan strains,Korea strains,Germany strains and Britain strains were in different branches, which could indicate that the prevalence of PSV in our country might be independent evolution.

Key words: porcine sapelovirus, epidemiological survey, 1B gene, genetic variation

CLC Number:

S855.3

LI Yu-meng, GUO Bo, XU Yi-Fei, CHEN Ying-qi, CAI Yao, LI Xiao-Jing, GONG Shuang-yan, XU Zhi-wen, ZHU Ling. Epidemiological survey of porcine sapelovirus and genetic variation analysis of 1B gene in Sichuan Province[J]. , 2016, 28(12): 1992-1999.

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Epidemiological survey of porcine sapelovirus and genetic variation analysis of 1B gene in Sichuan Province

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