Microbial ecological process of nitrogen transformation accelerated by different organic carbon in polluted water

doi:10.3969/j.issn.1004-1524.2016.11.17

›› 2016, Vol. 28 ›› Issue (11): 1915-1921.DOI: 10.3969/j.issn.1004-1524.2016.11.17

• Environmental Science • Previous Articles Next Articles

Microbial ecological process of nitrogen transformation accelerated by different organic carbon in polluted water

ZHAO Pei-wen^{1, 2}, WANG Xin², WU Yi-fei², YAO Xiao-hong², LIU Yong², SUN Hong², GE Xiang-yang¹, TANG Jiang-wu^{2, *}

1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070,China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China

Received:2016-03-14 Online:2016-11-15 Published:2016-11-16

Abstract

Abstract: In the present study, methanol, ethanol, sodium acetate, sodium citrate, glucose, sucrose, maltose, and CMC-Na was added in nitrogen-polluted urban river water with C/N of 15∶1. Variation of ammonia, nitrite, nitrate, total nitrogen were detected by national standard method, β-glucosidase activity were determined using the fluorogenic model substrates, and the change of bacterial community structure were analyzed by PCR-DGGE. It was shown that contents of ammonia decreased significantly after organic carbon sources addition except methanol or CMC-Na. Addition of organic carbon sources increased β-glucosidase activity. Bacterial community structure differed obviously with addition of glucose, sucrose and maltose. The results indicated that addition of organic carbon could accelerate transformation of nitrogen in the nitrogen-polluted water, in which microbes played key role. Bacterial community and metabolic activity had different responses to varied organic carbons, which would lead to different strength on nitrogen transformation acceleration.

Key words: organic carbon, polluted water, bacterial community, PCR-DGGE, bacterial metabolic activity

CLC Number:

Q938.1

ZHAO Pei-wen, WANG Xin, WU Yi-fei, YAO Xiao-hong, LIU Yong, SUN Hong, GE Xiang-yang, TANG Jiang-wu. Microbial ecological process of nitrogen transformation accelerated by different organic carbon in polluted water[J]. , 2016, 28(11): 1915-1921.

References

[1] WU J, YAN G, ZHOU G, et al. Model predictive control of biological nitrogen removal via partial nitrification at low carbon/nitrogen (C/N) ratio[J]. Journal of Environmental Chemical Engineering , 2014, 2(4):1899-1906.
[2] SANTOS C E D, MOURA R B, DAMIANOVIC M H R Z, et al. Influence of COD/N ratio and carbon source on nitrogen removal in a structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA)[J]. Journal of Environmental Management , 2015, 166:519-524.
[3] 刘刚, 闻岳, 周琪. 人工湿地反硝化碳源补充研究进展[J]. 水处理技术, 2010, 36(4):1-5.
LIU G, WEN Y, ZHOU Q. Advance in enhancement of denitrification in the constructed wetlands using external carbon sources[J]. Technology of Water Treatment , 2010, 36(4): 1-5. (in Chinese with English abstract)
[4] KOCATURK I, ERGUDER T H. Influent COD/TAN ratio affects the carbon and nitrogen removal efficiency and stability of aerobic granules[J]. Ecological Engineering , 2016, 90:12-24.
[5] INGERSOLL T L, BAKER L A. Nitrate removal in wetland microcosms[J]. Water Research , 1998, 32(3):677-684.
[6] 罗佳, 韩士群, 罗海荣,等. 外加碳源对富营养化水体生物脱氮效果及细菌群落结构的影响[J]. 江苏农业学报, 2012, 28(6):1312-1317.
LUO J, HAN S Q, LUO H R, et al. Effect of exogenus carbon resource on biological denitrification and bacterial community structure of eutrophic waters[J]. Jiangsu Journal of Agricultural Sciences , 2012, 28(6): 1312-1317. (in Chinese with English abstract)
[7] 裴廷权, 王波, 刘欢. 固体缓释碳源处理低碳氮比污水的脱氮及机理[J]. 环境工程学报, 2014, 8(6):2423-2428.
PEI T Q, WANG B, LIU H. Nitrogen removal and mechanism of low C/N wastewater processed by solid sustain-released carbon[J]. Chinese Journal of Environmental Engineering , 2014, 8(6): 2423-2428. (in Chinese with English abstract)
[8] 肖蕾, 贺锋, 黄丹萍,等. 人工湿地反硝化外加碳源研究进展[J]. 水生态学杂志, 2012, 33(1):139-143.
XIAO L, HE F, HUANG D P, et al. Research advances of adding extra carbon sources to denitrification for constructed wetlands[J]. Journal of Hydroecology , 2012, 33(1): 139-143. (in Chinese with English abstract)
[9] 丁怡, 王玮, 王宇晖,等. 不同进水碳氮比对水平潜流人工湿地脱氮效果的影响[J]. 工业水处理, 2014, 34(10):29-32.
DING Y, WANG W,WANG Y H, et al. Effects of C/N ratio of different kinds of influent on the nitrogen removing effectiveness in horizontal subsurface flow constructed wetland[J]. Industrial Water Treatment , 2014, 34(10): 29-32. (in Chinese with English abstract)
[10] XIN W, LI Z, SU J, et al. Lysis of a red-tide causing alga, Alexandrium tamarense , caused by bacteria from its phycosphere[J]. Biological Control , 2010, 52(2):123-130.
[11] 王新, 李志江, 郑天凌. 海洋浮游细菌在东海赤潮高发区的分布与活性[J]. 环境科学, 2010, 31(2):287-295.
WANG X, LI Z J, ZHENG T L, et al. Distribution and activity of marine bacterioplankton at frequent HAB area of East China Sea[J]. Chinese Journal of Environmental Science , 2010, 31(2): 287-295. (in Chinese with English abstract)
[12] MUYZER G, WAAL E C D, UITTERLINDEN A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J]. Applied & Environmental Microbiology , 1993, 59(3):695-700.
[13] PROSSER J I. The ecology of nitrifying bacteria[M]// BOTHE H, FERGUSON S J, NEWTON W E. Biology of the nitrogen cycle. Amsterdam: Elsevier Publishers, 2007: 407-427.
[14] MANDER Ü, MADDISON M, SOOSAAR K, et al. The impact of pulsing hydrology and fluctuating water table on greenhouse gas emissions from constructed wetlands[J]. Wetlands , 2011, 31(6):1023-1032.
[15] VYMAZAL J, MASA M. Horizontal sub-surface flow constructed wetland with pulsing water level[J]. Water Science & Technology , 2003, 48(5):143-148.
[16] 赵联芳, 朱伟, 赵建. 人工湿地处理低碳氮比污染河水时的脱氮机理[J]. 环境科学学报, 2006, 26(11):1821-1827.
ZHAO L F, ZHU W, ZHAO J. Nitrogen removal mechanism in constructed wetland used for treating polluted river water with lower ratio of carbon to nitrogen[J]. Acta Scientiae Circumstantiae , 2006, 26(11): 1821-1827. (in Chinese with English abstract)
[17] RUSTIGE H, NOLDE E. Nitrogen elimination from landfill leachates using an extra carbon source in subsurface flow constructed wetlands[J]. Water Science & Technology , 2007, 56(3):125-133.
[18] HUME N P, FLEMING M S, HORNE A J. Plant carbohydrate limitation on nitrate reduction in wetland microcosms[J]. Water Research , 2002, 36(3):577-584.
[19] BASTVIKEN S K, ERIKSSON P G, PREMROV A, et al. Potential denitrification in wetland sediments with different plant species detritus[J]. Ecological Engineering , 2006, 25(2):183-190.

Microbial ecological process of nitrogen transformation accelerated by different organic carbon in polluted water

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XIA Wenjian, QIN Wenjing, LIU Jia, CHEN Xiaofen, ZHANG Lifang, CAO Weidong, XU Changxu, CHEN Jingrui. Vertical distribution of soil organic carbon and dissolved organic carbon in reddish paddy soil under long-term green manure utilization [J]. , 2020, 32(5): 878-885.
[2]	JIAN Xing, ZHAI Xiaoyu, WANG Yu, CAI Yangyang. Influence of land use changes on soil total organic carbon and dissolved organic carbon in wetland [J]. , 2020, 32(3): 475-482.
[3]	LIAN Yuzhen, LIU Heman, CAO Lihua, HAN Xiaohao, MA Heping. Distribution of soil aggregate and organic carbon under different land use types in Linzhi, Tibet [J]. , 2019, 31(8): 1353-1360.
[4]	GUAN Qinzhuang, CHENG Yongxu, LI Cong, WANG Haifeng, CHEN Huangen, LI Jiayao. Changes of soil organic carbon and relationships with soil properties in rice-crayfish coculture system [J]. , 2019, 31(1): 113-120.
[5]	TAO Libo, WANG Jianjun, WANG Guohui, YU Shuang, LI Huihui, XU Dongmei. Effects of enclosure on soil organic carbon mineralization of desert steppe in Ningxia [J]. , 2017, 29(9): 1549-1554.
[6]	YU Zhoulu, QIU Lefeng, LIN Lin. Influence of land use changes on soil organic carbon distribution in agricultural soils [J]. , 2017, 29(5): 806-811.
[7]	JI Bo, LI Na, MA Fan, CAI Jinjun, DONG Liguo, XU Hao, HAN Xinsheng. Effect of typical re-vegetation patterns on soil organic carbon sequestration in southern Ningxia [J]. , 2017, 29(3): 483-488.
[8]	ZHANG Zhichao, SUN Hong, WU Yifei, WANG Xin, YAO Xiaohong, LIU Yong, TANG Jiangwu, GE Xiangyang. PCR-DGGE assisted selection of ammonia degrading bacteria [J]. , 2017, 29(2): 286-291.
[9]	WANG Xin, CHENG Liang. Soil bacterial community composition and diversity of five soil types in Qinghai-Tibetan Plateau [J]. , 2017, 29(11): 1882-1889.
[10]	LAN Jiacheng, XIAO Shizhen, LIN Junqing, SHEN Yan. Effect of land use types on soil light and heavy fraction organic carbon in Karst mountain area [J]. , 2017, 29(10): 1720-1725.
[11]	WANG Lianxiao1， SHI Zhengtao1，*， LIU Xinyou2，3， YANG Fan1. Distribution characteristics of soil organic carbon of rubber plantation in Xishuangbanna [J]. , 2016, 28(7): 1200-.
[12]	SHAO Yangfeng1， MEI Hongfei1， PAN zhongchao1， LIU Huan2， WANG Chaoqi2. Effects of corn straw returning on soil organic carbon content， microbial functional diversity and cabbage yield [J]. , 2016, 28(5): 838-.
[13]	JIAN Xing1，2， WANG Song3， WANG Yu\\|liang3， WANG Jian\\|fei1，2. Soil organic carbon and its active components characteristics under different land utilization types at the periphery of city wetlands [J]. , 2016, 28(1): 119-.
[14]	ZHANG Zhen\\|xing1，2， ZHANG Wen\\|zhao1， YANG Hui\\|cui1，2， CHEN Chun\\|lan1， WEI Wen\\|xue1，*. Effects of growing rice roots on the bacterial abundance and community structure in the rhizosphere during tillering stage [J]. , 2015, 27(12): 2045-.
[15]	ZHU Zhen\\|ling1，2， MA Wan\\|zhu2， LONG Wen\\|li2， REN Zhou\\|qiao2， DENG Xun\\|fei2， CHEN Xiao\\|jia2， SHEN Jian\\|guo3， LYU Xiao\\|nan1，2，*. Spatial distribution characteristics of topsoil organic carbon in farmland and its influencing factors in Yuhang District， Hangzhou City [J]. , 2015, 27(11): 1990-.