Effects of spraying organic and inorganic nitrogen on physiological characteristics of rice in dry cultivation

doi:10.3969/j.issn.1004-1524.2017.03.01

›› 2017, Vol. 29 ›› Issue (3): 353-359.DOI: 10.3969/j.issn.1004-1524.2017.03.01

• Crop Science • Next Articles

Effects of spraying organic and inorganic nitrogen on physiological characteristics of rice in dry cultivation

WU Meiyan

College of Agriculture, Yangtze University/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Jingzhou 434025, China

Received:2016-08-22 Online:2017-03-20 Published:2017-03-31

Abstract

Abstract: A potted culture experiment was conducted to study the effects of organic and inorganic nitrogen on physiological characteristics of rice in dry cultivation, the results would provide a scientific basis for the application of organic nitrogen in rice production and the development of organic fertilizer. The effect of spraying ammonium sulphate (AS), glycine (Gly) and glutamine (Glu) on the leaf surface of rice (Oryza sativa L., Liangyoupeijiu) in dry cultivation under drought stress at the booting stage was evaluated with water-saving management (WSM) and distilled water (DW) as the control, and the chlorophyll content, protective enzymes, osmotic regulation matters, yield and protein content of rice were measured. The results showed that chlorophyll content and yield were decreased, and content of malondialdehyde (MDA) was increased obviously compared to WSM (P<0.05). After spraying AS, Gly and Glu, the chlorophyll content, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) of flag leaves of rice in dry cultivation under drought stress were increased, and MDA content was decreased significantly. The osmotic regulators were enhanced, such as proline (Pro), total free amino acid (TFA) and the contents of soluble protein (SP). As a result, the yield and protein content of rice in dry cultivation were improved obviously (P<0.05). The increase of kernel numbers, 1000 grain yield and seed setting rate were the key point of harvesting higher yield for these treatments. Effects of AS and Gly were better than Glu, there was no significant difference between AS and Gly. Compared to DW, the yield and protein content of rice in dry cultivation were increased by 56.3% and 20.5% for AS and 44.2% and 22.0% for Gly. It suggested that spraying exogenous organic and inorganic nitrogen on rice in dry cultivation under drought stress could help rice keeping low peroxide contents and enhance rice resistance to drought by increasing activities of antioxidant enzymes and contents of osmotic regulators.

Key words: organic nitrogen, protective enzyme, osmotic regulation, rice in dry cultivation

CLC Number:

S511.6

WU Meiyan. Effects of spraying organic and inorganic nitrogen on physiological characteristics of rice in dry cultivation[J]. , 2017, 29(3): 353-359.

References

[1] 陈贵, 郭世伟, 赵国华, 等. 旱作对水稻生长及氮、磷利用效率的影响[J]. 干旱地区农业研究, 2015, 33(1): 182-186, 251.
CHEN G, GUO S W, ZHAO G H, et al. Effects of upland cultivation on rice grain yield and N & P use efficiency[J]. Agricultural Research in the Arid Areas , 2015, 33(1): 182-186, 251. (in Chinese with English abstract)
[2] HUANG J P, WANG B F, YANG X L, et al. Advance of rice drought resistance, water saving cultivation and genetic breeding[J]. Agricultural & Technology , 2016, 17(5): 1115-1119.
[3] 柏彦超, 倪梅娟, 王娟娟, 等. 水分胁迫对旱作水稻产量与养分吸收的影响[J]. 农业工程学报, 2007, 23(6): 101-104.
BO Y C, NI M J, WANG J J, et al. Effects of water stress on rice yields and nutrients absorption under aerobic condition[J]. Transactions of the Chinese Society of Agricultural Engineering , 2007, 23(6): 101-104. (in Chinese with English abstract)
[4] 路兴花, 吴良欢, 庞林江. 覆膜后土壤水分对水稻生物学特性和产量的影响[J]. 浙江农业学报, 2009, 21(5): 463-467.
LU X H, WU L H, PANG L J. Effects of soil moisture under plastic film mulching on the biological and yield characteristics of rice ( Oryza sativa L.)[J]. Acta Agricuhurae Zhejiangensis , 2009, 21(5): 463-467.
[5] 郑丽, 樊剑波, 何园球, 等. 不同供磷水平对旱作条件下水稻水稻生长、根系形态和养分吸收的影响[J]. 土壤, 2015, 47(4): 664-669.
ZHENG L, FAN J B, HE Y Q, et al. Effects of phosphorus on growth, phosphorus uptake and utilization efficiency of rice seedlings[J]. Soils , 2015, 47(4): 664-669. (in Chinese with English abstract)
[6] GRIFFITHS H, PARRY M A J. Plant responses to water stress[J]. Plant Biology , 1973, 89(24): 801-802.
[7] GARCIA A L, FUENTES V, GALLEGO J. Influence of nitrogen supply on osmoregulation in tomato ( Lycopersicon esculentum Mill.) plants under moderate water stress[J]. Plant Science , 1996, 115(1): 33-38.
[8] 李宪利, 高东升, 顾曼如, 等. 铵态氮和硝态氮对苹果植株SOD和POD活性的影响(简报)[J]. 植物生理学通讯, 1997, 33(4): 245-255.
LI X L, GAO D S, GU M R, et al. Effects of ammonium and nitrate nitrogen on the activities of superoxidase dismutase (SOD) and peroxidase (POD) of apple trees[J]. Plant Physiology Communication , 1997, 33(4): 245-255. (in Chinese)
[9] GAO Y X, LI Y, YANG X X, et al. Ammonium nutrition increases water absorption in rice seedlings ( Oryza sativa L.) under water stress[J]. Plant and Soil , 2010, 331(1): 193-201.
[10] LI H J, LI Y, YANG X X, et al. Effects of different nitrogen forms and water stress on the growth and osmotic adjustment of rice seedlings[J]. Chinese Journal of Rice Science , 2010, 24(4): 403-409.
[11] CHANG Z H, LIU Y, DONG H, et al. Effects of cytokinin and nitrogen on drought tolerance of creeping bentgrass[J]. PLoS One , 2016, 11(4): 1-19.
[12] VALLIERE J M, ALLEN E B. Interactive effects of nitrogen deposition and drought-stress on plant-soil feedbacks of Artemisia californica seedlings[J]. Plant and Soil , 2016, 403(1): 277-290.
[13] 高迎旭, 周毅, 郭世伟, 等. 不同形态氮素营养对水稻抗旱性影响的研究[J]. 干旱区研究, 2006, 23(4): 598-603.
GAO Y X, ZHOU Y, GUO S W, et al. Effects of different nitrogen supply forms on drought resistance of paddy plants[J]. Arid Zone Research , 2006, 23(4): 598-603. (in Chinese with English abstract)
[14] 周秀杰, 王海红, 束良佐, 等. 局部根区水分胁迫下氮形态与供给部位对玉米幼苗生长的影响[J]. 应用生态学报, 2010, 21(8): 2017-2024.
ZHOU X J, WANG H H, SU L Z, et al. Effects of nitrogen form and its supply position on maize seedling growth under partial root-zone water stress[J]. Chinese Journal of Applied Ecolog y, 2010, 21(8): 2017-2024. (in Chinese with English abstract)
[15] 周秀杰, 王海红, 束良佐, 等. 供氮形态与部位对局部根区水分胁迫下玉米水分吸收与利用的影响[J]. 西北植物学报, 2010, 30(7): 1426-1434.
ZHOU X J, WANG H H, SU L Z, et al. Water uptake and utilization of maize under partial root-zone water stress with different nitrogen forms and application position[J]. Acta Botanica Boreali-Occidentalia Sinica , 2010, 30(7): 1426-1434. (in Chinese with English abstract)
[16] 王海红, 束良佐, 周秀杰, 等. 固定根区水分胁迫下氮形态对玉米幼苗水分利用的调节与作用机制[J]. 中国农学通报, 2009, 25(18):155-160.
WANG H H, SU L Z, ZHOU X J, et al. Regulation and the mechanisms of nitrogen form on water utilization of maize seedlings under fixed partial root-zone water stress[J]. Chinese Agricultural Science Bulletin , 2009, 25(18): 155-160. (in Chinese with English abstract)
[17] 王熹, 陶龙兴, 黄效林, 等. 灌溉稻田水稻旱作技术要素及产量形成[J]. 中国农业科学, 2004, 37(4): 502-509.
WANG J, TAO L X, HUANG X L, et al. Study on non-blooding farming technique in paddy field: technique specification and formation of yield components[J]. Scientia Agricultura Sinica , 2004, 37(4): 502-509. (in Chinese with English abstract)
[18] 苍晶, 赵会杰. 植物生理学实验教程[M]. 北京: 高等教育出版社, 2013.
[19] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[20] 吴建国, 石春海, 张小明, 等. 用近红外反射光谱法分析稻米3种必需氨基酸含量的研究[J]. 作物学报, 2003, 29(5): 688-692.
WU J G, SHI C H, ZHANG X M, et al. Analysis of three types of essential amino acids content in milled rice by near infrared reflectance spectroscopy[J]. Acta Agronomica Sinica , 2003, 29(5): 688-692. (in Chinese with English abstract)
[21] 曹翠玲, 李生秀, 张占平. 氮素形态对小麦生长中后期保护酶等生理特性的影响[J]. 土壤通报, 2003, 34(4): 295-298.
CAO C L, LI S X, ZHANG Z P. Effects of N form on the activity of protectiase and wheat yield at the vegetative and reproductive growth stage[J]. Chinese Journal of Soil Science , 2003, 34(4): 295-298. (in Chinese with English abstract)
[22] 朱维琴, 吴良欢, 陶勤南. 不同氮营养对干旱逆境下水稻生长及抗氧化性能的影响研究[J].植物营养与肥料学报, 2006, 12(4): 506-510.
ZHU W Q, WU L H, TAO Q N. Effects of different nitrogen nutrition on the growth and antioxidant characteristics of rice ( Oryza sativa L) stressed by drought[J]. Plant Nutrition and Fertility Science , 2006, 12(4): 506-510. (in Chinese with English abstract)
[23] 张军, 刘红, 李晓萍, 等. 干旱对小麦孕穗期叶片生理特性及产量的影响[J].干旱地区农业研究, 2014, 32(3): 1-8.
ZHANG J, LIU H, LI X P, et al. Effects of drought on leaf physiological parameters and yield of wheat at booting stage[J]. Agricultural Research in the Arid Areas , 2014, 32(3): 1-8. (in Chinese with English abstract)
[24] 姜慧芳, 任小平. 干旱胁迫对花生叶片SOD活性和蛋白质的影响[J].作物学报, 2004, 30(2): 169-174.
JIANG H F, REN X P. The effect on SOD activity and protein content in groundnut leaves by drought stress[J]. Acta Agronomica Sinica , 2004, 30(2): 169-174. (in Chinese with English abstract)
[25] 栗海俊, 李勇, 杨秀霞, 等. 不同形态氮素营养和水分条件对苗期水稻生长及渗透调节能力的影响[J]. 中国水稻科学, 2010, 24(4): 403-409.
LI H J, LI Y, YANG X X, et al. Effects of different nitrogen forms and water stress on the growth and osmotic adjustment of rice seedlings[J]. Chinese Journal of Rice Research , 2010, 24(4): 403-409. (in Chinese with English abstract)
[26] 高焕晔, 王三根, 宗学凤, 等. 灌浆结实期高温干旱复合胁迫对稻米直链淀粉及蛋白质含量的影响[J]. 中国生态农业学报, 2012, 20(1): 40-47.
GAO H Y, WANG S G, ZONG X F, et al. Effects of combined high temperature and drought stress on amylose and protein contents at rice grain-filling stage[J]. Chinese Journal of Eco-Agriculture , 2012, 20(1): 40-47. (in Chinese with English abstract)
[27] 卢红芳, 王晨, 郭天财, 等. 灌浆前期高温和干旱胁迫对小麦籽粒蛋白质含量和氮代谢关键酶活性的影响[J]. 生态学报, 2014, 34(13): 3612-3619.
LU H F, WANG C, GUO T C, et al. Effects of high-temperature and drought stress on protein concentration and key enzyme activities in relation to nitrogen metabolism in wheat grains during the early stage of grain filling[J]. Acta Ecologica Sinica , 2014, 34(13): 3612-3619. (in Chinese with English abstract)
[28] 陈亮. 干旱胁迫对水稻叶片光合作用和产量及稻米品质的影响研究[D]. 武汉: 华中农业大学, 2015.
CHEN L. The effect of drought stress on rice leaf’s photosynthesis, rice grain yield and rice quality [D]. Wuhan: Huazhong Agricultural University, 2015. (in Chinese with English abstract)
[29] 曹小闯, 吴良欢, 马庆旭, 等. 高等植物对氨基酸氮的吸收与利用研究进展[J]. 应用生态学报, 2015, 26(3): 919-929.
CAO X C, WU L H, MA Q X, et al. Advances in studies of absorption and utilization of animal acids by plants[J]. Chinese Journal of Applied Ecology , 2015, 26(3): 919-929. (in Chinese with English abstract)
[30] 吴良欢, 陶勤南. 水稻氨基酸氮营养效应及其机理研究[J]. 土壤学报, 2000,37(4): 464-473.
WU L H, RAO Q N. Effects of amino acid-N on rice nitrogen nutrition and its mechanism[J]. Acta Pedologica Sinica , 2000, 37(4): 464-473. (in Chinese with English abstract)

Effects of spraying organic and inorganic nitrogen on physiological characteristics of rice in dry cultivation

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