Promotion effect of fungus DL12 fermentation liquid on salt-resistance ability of tomato and primary identification of strain

doi:10.3969/j.issn.1004-1524.2017.04.13

›› 2017, Vol. 29 ›› Issue (4): 605-610.DOI: 10.3969/j.issn.1004-1524.2017.04.13

• Horticultural Science • Previous Articles Next Articles

Promotion effect of fungus DL12 fermentation liquid on salt-resistance ability of tomato and primary identification of strain

LIU Guoli¹, GONG Na¹, CHEN Xun¹, WANG Na^{2, *}

1.Center of Microbial Engineering, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China;
2. Institute of Plant Nutrients and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China

Received:2016-12-21 Online:2017-04-20 Published:2017-04-27

Abstract

Abstract: In order to screen fungi that could improve the salt-resistance ability of Lycopersicon esculentum, firstly, the fermentation liquid of different fungi were used to treat the seeds of Lycopersicon esculentum under 75 mmol·L^-1 Ca(NO₃)₂ stress. Then the seedlings were sprayed by fermentation liquid of different fungi which had good effects on germination of seeds through evaluation of germination rate and germination potential. The plant height and biomass of seedlings after treatment were recorded. Effect of fermentation liquid from strains DL2, DL12, DL15, DWJ2, DWR3 on endogenous hormones content of seedlings were also measured through HPCE chromatograms. Results showed that several fungi strains could partially improve germination rate and germination potential of seeds, and increase the dry weight. DL12 had the best effects among all the fungi. The germination rate, germination potential of seeds, plant height, root dry weight, and seedling dry weight were increased by 29%, 200%, 17.6%, 26.8%, 18.9%, respectively, compared with the control group. The IAA content was 49.6 times of the control group. The fungus general primers ITS1 and ITS2 were used to amplify 18S rDNA sequences of strain DL12, and alignment with the known sequences from GenBank was carried out, results showed that the similarity between strain DL12 and Schizophyllum commune was 99%. It was concluded that DL12 may belong to Schizophyllum sp.

Key words: tomato, salt-resistance, fungus, fermentation liquid

CLC Number:

S182

LIU Guoli, GONG Na, CHEN Xun, WANG Na. Promotion effect of fungus DL12 fermentation liquid on salt-resistance ability of tomato and primary identification of strain[J]. , 2017, 29(4): 605-610.

References

[1] 顾京晏,顾卫,张化,等.我国设施农业土壤次生盐渍化生物改良措施研究进展[J].北京师范大学学报(自然科学版),2016,52(1):70-75.
GU J Y, GU W, ZHANG H, et al. Biological measures for soil improvement of facility agriculture in China[J]. Journal of Beijing Normal University ( Nature Science ), 2016,52(1):70-75.(in Chinese with English abstract)
[2] 王学征.设施环境盐胁迫对番茄生长发育及膜系统影响的研究[D].哈尔滨:东北农业大学,2004.
WANG X Z. Studies on effects of salt stress on growth and development and membranes system of tomato in protected farm[D].Harbin: Northeast Agricultural University. 2004. (in Chinese with English abstract)
[3] PARIDA A K, DAS A B. Salt tolerance and salinity effects on plants: a review.[J]. Ecotoxicology and Environmental Safety , 2005, 60(3):324.
[4] 顾金凤.微生物菌肥对盐渍化土壤的改良研究[D].扬州:扬州大学,2013.
GU J F. Effects of microbiological fertilizer on salinized soil [D].Yangzhou: Yangzhou University, 2013. (in Chinese with English abstract)
[5] 黄红艳.次生盐渍化土壤的微生物多样性及微生物改良效应研究[D].上海:上海交通大学,2012.
HUANG H Y. Soil microbial diversity in secondary salinization soils and it’s microbial remidation [D]. Shanghai: Shanghai Jiao Tong University, 2012. (in Chinese with English abstract)
[6] 马晓颖, 贾东贝, 李冰宇,等. 利用高效毛细管电泳测定真菌代谢产物吲哚乙酸含量[J].江苏农业科学, 2011, 39(3):439-441.
MA X Y, JIA D B, LI B Y, et al. Content determination of IAA in metabolic of fungi using high-performance capillary electrophoresis [J]. Jiangsu Agricultural Sciences , 2011, 39(3):439-441. (in Chinese with English abstract)
[7] 陶龙兴.内源IAA对亚种间杂交稻籽粒灌浆的信息效应[D].北京:中国农业科学院,2003.
TAO L X. Signaling effects of endogenous IAA on grain filling of subspecies hybrid rice[D]. Beijing: Chinese Academy of Agricultural Sciences, 2003. (in Chinese with English abstract)
[8] 尹培培, 包日双, 戴佳锟,等. 植物激素对植物器官发生影响的研究进展[J].江西农业学报,2012,24(6):37-41.
YIN P P, BAO R S, DAI J K, et al. Research advance in impact of phytohormone on plant organogenesis[J]. Acta Agriculturae Jiangxi , 2012, 24(6):37-41. (in Chinese with English abstract)
[9] 辛树权,王贵,高扬.植物生长促生菌对盐胁迫下水稻种子萌发及幼苗生长的影响[J]. 湖北农业科学,2012,51(3):490-496.
XIN S Q, WANG G, GAO Y. Effects of plant growth-promoting rhizobacteria (PGPR) on the rice germination and seedling development under salt stress [J]. Hubei Agricultural Sciences , 2012, 51(3):490-496. (in Chinese with English abstract)
[10] 郑元元, 岳海涛, 石在强, 等.盐胁迫下解盐促生细菌Rs-5和Rs-198促进棉花种子发芽的机理探讨[J].中国农业科学,2008,41(5):1326-1332.
ZHENG Y Y,YUE H T, SHI Z Q, et al. Physiochemical characters and ability to promote cotton germination of bacteria strains Rs-5 and Rs-198 under salt stress [J]. Scientia Agricultura Sinica , 2008, 41(5):1326-1332. (in Chinese with English abstract)
[11] 罗欢. 芽孢杆菌对植物的促生和耐盐作用及其相关机制的研究[D]. 南京:南京农业大学, 2013.
LUO H. Study of growth promoting and salt tolerance of Bacillus spp. on plant and the research of underlying mechanisms[D].Nanjing: Nanjing Agricultural University,2013. (in Chinese with English abstract)
[12] 吉云秀,黄晓东.植物促生菌对燕麦初生苗盐分胁迫下的促生效应[J].大连海事大学学报, 2007,33(2):86-89.
JI Y X, HUANG X D. Effects of plant growth-promoting rhizobacteria on the growth of oat seedlings under salt stress[J]. Journal of Dalian Maritime University , 2007, 33(2):86-89. (in Chinese with English abstract)
[13] 马晓敏,张红娟,顾沐宇,等. 促生菌的分离鉴定及其对柳枝稷种子在盐胁迫下的萌发促进作用[J].家畜生态学报,2015, 36(6):46-52.
MA X M, ZHANG H J, GU M Y, et al. Isolation and identification of plant growth-promoting Rhizobacteria(PGPR) and its promoting effect on switchgrass seed under NaCl salt stress[J]. Acta Ecologiae Animalis Domastici , 2015, 36(6):46-52. (in Chinese with English abstract)
[14] 王斌. 丛枝菌根真菌提高加工番茄抗盐和抗旱效能及其机理研究[D].石河子:石河子大学,2013.
WANG B. Arbuscular mycorrhizal fungi improve salt and drought resistance to processing tomato and study of their mechanisms [D]. Shihezi: Shihezi University, 2013. (in Chinese with English abstract)
[15] 李娇. 碱蓬内生菌对Na 2 CO 3 胁迫下水稻幼苗生长的缓解作用[D].沈阳:沈阳师范大学,2014.
LI J. Effect of endophyte infection alleviate growth damage of rice seedlings under Na 2 CO 3 stress [D]. Shenyang: Shenyang Normal University, 2014. (in Chinese with English abstract)
[16] 李莎.外生菌根真菌和荧光假单胞菌对油松生长和抗病性的影响[D].杨凌:西北农林科技大学,2011.
LI S. Effects of ectomycorrhiza fungi and Pseudomonas fluorescens to Pinus tabulaeformis growth and disease resistance[D]. Yangling: Northwest A&F University, 2011. (in Chinese with English abstract)

Promotion effect of fungus DL12 fermentation liquid on salt-resistance ability of tomato and primary identification of strain

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