Abstract: With the commercialization of genetically modified organism (GMO) and large scale of rapeseed imported to China, it was essential to manage and control existing risks. In this paper, genetically modified rapeseed Ms8 was prepared including analysis on preparation, purity, homogeneity, stability and certified value. The results showed that the homogeneity of endogenous gene and exogenous gene of reference molecule was well. No other selective specific gene had been amplified by PCR. Short term stability of reference molecule was obviously changed which were stored at room temperature for 14 days, and long term stability of reference molecule was more than 6 months. The certified value of exogenous gene of reference molecule determined by microspectrophotometric method and digital PCR method was similar. Thus, pMs8 can be used as reference material, and the reference molecule is suitable for the rapid identification of modified rapeseed Ms8 in unknown samples.

Key words: transgenic rapeseed, digital PCR, reference plasmid, Ms8