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Effect of compound Chinese medicine preparation on the antioxidant function in serum of SD rats
ZHOU Jinxing, JIN Erhui, LIU Wenju, ZHANG Chaochao, WANG Sifei, JIN Guangming
2017, 29(5):
737-743.
DOI: 10.3969/j.issn.1004-1524.2017.05.08
To study the effects of compound traditional Chinese medicine preparation, which consists of Radix astragali, Radix isatidis, Fried atractylodes, Poria cocos, orange peel, hawthorn, liquorice and yam on the T-AOC, T-SOD, GSH-Px activity and MDA content in serum of SD rats. 150 SD rats (SPF animal) were randomly divided into control group, 1.5, 3.0 and 6.0 mL·kg-1 treatment group with 15, 45, 45, 45 SD rats, respectively. The rats of different treatment groups were administered using gavage methods according to dosage 3.0 mL·kg-1 distilled water for control group, and 1.5, 3.0 and 6.0 mL·kg-1 compound traditional Chinese medicine preparation (containing raw herbs 1 g·mL-1) for treatment groups, respectively. Rats were regularly weighed and given medicine or distilled water by gavage every day. On the 10th, 30th and 60th day of the experiment, 5 rats fasted and banned water for 10 h were selected in every experiment group to be weighed and picked blood from the heart, for isolating serum sample to measure T-AOC, T-SOD, GSH-Px activities and MDA content. The results showed that on the experimental 10th, 30th and 60th day, the T-AOC activity in 3.0 mL·kg-1 and 6.0 mL·kg-1 group significantly (P<0.05) or extremely significantly increased (P<0.01), and the MDA contents in 3.0 mL·kg-1 and 6.0 mL·kg-1 group significantly (P<0.05) or extremely significantly decreased (P<0.01), compared with the control group on the same day. On the 30th and 60th day, the T-SOD activity in 3.0 mL·kg-1 group significantly increased (P<0.05), and the GSH-Px activity in 6.0 mL·kg-1 group significantly increased (P<0.05), compared with the control group. Compound traditional Chinese medicine preparation could significantly improve the SD rats' antioxidant function. The optimum dosage of compound traditional Chinese medicine preparation to rats was 3.0 mL·kg-1 by gavage methods.
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