Influence of noise on HSPs mRNA transcription in internal organs of meat duck

doi:10.3969/j.issn.1004-1524.2017.11.04

›› 2017, Vol. 29 ›› Issue (11): 1796-1799.DOI: 10.3969/j.issn.1004-1524.2017.11.04

• Animal Scince • Previous Articles Next Articles

Influence of noise on HSPs mRNA transcription in internal organs of meat duck

ZHANG Yang¹, CHEN Bowen¹, BIAN Youqing², WANG Zhaoshan³, XU Qi¹, CHANG Guobin¹, CHEN Guohong^{1, *}

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Modern Animal Husbandry Science and Technology Park, Taizhou 225300, China;
3. Jiangsu Eco Food Co., Ltd, Suqian 223800, China

Received:2017-04-01 Online:2017-11-20 Published:2017-12-05

Abstract

Abstract: In order to clarify the physiological effects of noise on meat ducks and improve the animal welfare of meat ducks, 60 ducks were randomly divided into 3 groups. The 80 and 100 dB noise were applied by noise generator to establish stress model in experimental group 1 and 2, respectively. The control group did not apply noise stress. Liver, spleen, lung and kidney tissues were collected after the stress treatment. RT-PCR was used to detect the expression of HSP70 and HSP90 mRNA in each tissue. The results showed that transcription levels of HSP70 and HSP90 mRNA in the liver, spleen, lung and kidney of experimental group 1 and 2 were significantly higher than those in the control group (P<0.01), and the transcription levels of HSP70 and HSP90 mRNA in experimental group 2 were significantly (P<0.01) higher than those in experimental group 1. Therefore, acute noise stress would increase the transcript of HSPs mRNA in the internal organs of meat ducks.

Key words: noise stress, meat duck, HSP70, HSP90

CLC Number:

S834

ZHANG Yang, CHEN Bowen, BIAN Youqing, WANG Zhaoshan, XU Qi, CHANG Guobin, CHEN Guohong. Influence of noise on HSPs mRNA transcription in internal organs of meat duck[J]. , 2017, 29(11): 1796-1799.

References

[1] ALGERS B, EKESBO I, SROMBERG S. The impact of continuous noise on animal health[J]. Acta Veterinaria Scandinavica Supplementum , 1978, 19(67):1-26.
[2] WAYNERT D F, STOOKEY J M, SCHWARTZKOPF-GENSWEIN K S, et al. The response of beef cattle to noise during handling[J]. Applied Animal Behaviour Science , 1999, 62(1):27-42.
[3] MOLINO, J A. Annoyance and noise [M]// HARRIS C M. Handbook of noise control [M]. [S.l.]: McGraw-Hill, 1979, 26(4):41-51.
[4] MELAMED S, BRUHIS S. The effects of chronic industrial noise exposure on urinary cortisol, fatigue and irritability: a controlled field experiment[J]. Journal of Occupational & Environmental Medicine , 1996, 38(3):252-256.
[5] 王同国,史斌. 热休克蛋白调控的研究进展[J]. 现代免疫学,2007,27(1):73-76.
WANG T G, SHI B. The research progress of heat shock protein regulation[J]. Modern Immunology , 2007, 27(1):73-76. (in Chinese)
[6] 万莉莉,胡明华,史绍蓉,等. 运动应激状态下的心肌热休克蛋白表达[J]. 中国组织工程研究,2014,18(38):6216-6221.
WAN L L, HU M H, SHI S R, et al. Expression of myocardial heat shock protein under stress state[J]. Chinese Journal of Tissue Engineering Research , 2014, 18(38):6216-6221. (in Chinese with English abstract)
[7] 罗靖旻,罗建新. 热休克蛋白免疫作用的研究进展[J]. 医学综述,2013,19(7):1179-1181.
LUO J M, LUO J X.Research progress of the immunity of heat shock proteins[J]. Medical Recapitulate , 2013, 19(7):1179-1181.(in Chinese with English abstract)
[8] 许生友,陈兴勇,姜润深,等. 肉鸡热应激下肝脏和下丘脑HSP70 mRNA的表达[J]. 中国农学大学学报,2011,6(3):112-116.
XU S Y, CHEN X Y, JIANG R S, et al. Expression of HSP70 mRNA in liver and hypothalamus in chicken under heat stress[J]. Journal of China Agricultural University , 2011, 6(3):112-116. (in Chinese with English abstract)
[9] 计红,吴永魁,胡仲明,等. 不同强度冷应激下仔猪淋巴细胞HSP70 mRNA的转录规律[J]. 应用与环境生物学报,2007,13(4) :507-509.
JI H, WU Y K, HU Z M, et al. HSP70 mRNA transcription in lymphocyte of piglets under cold stress with different intensities[J]. Chinese Journal of Applied and Environmental Biology , 2007, 13(4) :507-509. (in Chinese with English abstract)
[10] JI H, WU Y K, GUO J R, et al. Effects of cold stress on expression of Hsp 70 gene in blood lymphocyte of piglets[J]. Journal of Animal & Veterinary Advances , 2012, 11(21):3914-3920.
[11] MOSSER D D, CARON A W, BOURGET L, et al. Role of the human heat shock protein HSP70 in protection against stress-induced apoptosis[J]. Molecular and Cellular Biology , 1997, 17(9):5317-5327.
[12] TANG M T, SUN M, LU M X, et al. Expression patterns of five heat shock proteins in Sesamia inferens (Lepidoptera: Noctuidae) during heat stress[J]. Journal of Asia-Pacific Entomology , 2015, 18(3):529-533.
[13] 雷蕾. 肉鸡组织的热应激损伤与HSP90水平及其mRNA表达的变化 [D]. 南京:南京农业大学,2008:9-15
LEI L. Corresponding mRNA in the heat-stressed broilers [D]. Nanjing: Nanjing Agricultural University, 2008:9-15. (in Chinese with English abstract)

Influence of noise on HSPs mRNA transcription in internal organs of meat duck

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	YU Zonggang, JIANG Jun, YAO Yaling, GUO Ying, LI Chuang, YAN Haifeng. Correlation analysis of HSP70 gene polymorphism and semen quality in Xuefeng Black-bone chicken [J]. , 2020, 32(8): 1378-1384.
[2]	ZHANG Xiang\\|ying1，2， SHI Hong\\|xia1， DI He\\|shuang2，WANG Gen\\|lin1. Effects of glutamine on the growth， proliferation and expression of HSP70 in bovine mammary epithelial cells#br# [J]. , 2015, 27(9): 1535-.
[3]	JIA Feng;LIU Yan;WEI Qiang;XIAO Chenwen;JI Quanan;LI Ke;BAO Guolian;*. Effect of coated sodium butyrate on intestinal mucosa morphology and digestive enzyme activities in meat duck [J]. , 2014, 26(3): 0-570575.
[4]	ZENG Tao;LI Guo-qin;WANG De-qian;LI Jin-jun;TIAN Yong;CHEN Li;HUANG Ai-xia;SHI Fang-xiong;;SHEN Jun-da;TAO Zheng-rong;LU Li-zhi;. Single nucleotide polymorphisms （SNPs） of HSP70 gene segments and their association with laying performance of domestic duck [J]. , 2013, 25(2): 0-234.
[5]	ZUO Lan;YAN Qi-gui;*;LIU Bin;LI Shuang-jiang . Construction of eukaryotic recombinant expression vector containing Hsp70 and PRRSV ORF5, ORF6 genes and its responses in Cos-7 cell line [J]. , 2011, 23(3): 0-499.
[6]	ZHOU Bo;LI Bo-sheng*. Cloning of a putative heat shock protein 90 gene from Spirulina innermongoliansis and construction of three pro-integration expression vector [J]. , 2011, 23(2): 0-303.
[7]	ZOU Li—li;TAO Zheng-rong;SHEN Jun-da;REN Jin—dong;SHI Fang-xiong;;LU Li-zhi;. Efects of acute heat stress on expressional patterns of the heat shock protein 7O in the duck internal organs [J]. , 2009, 21(4): 0-334.