Identification of linear B cell epitope for E protein of duck Tembusu virus

doi:10.3969/j.issn.1004-1524.2017.12.08

›› 2017, Vol. 29 ›› Issue (12): 2009-2014.DOI: 10.3969/j.issn.1004-1524.2017.12.08

• Animal Science • Previous Articles Next Articles

Identification of linear B cell epitope for E protein of duck Tembusu virus

ZHANG Lin^{1, 2}, YU Bin^{1, *}, NI Zheng¹, CHEN Liu¹, YUN Tao¹, YE Weicheng¹, HUA Jionggang¹, CUI Yanshun², ZHANG Cun¹

1.Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2 College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China

Received:2016-08-22 Online:2017-12-20 Published:2018-01-08

Abstract

Abstract: This experiment was conducted to identify B cell epitope of E protein of duck Tembusu virus (DTMUV). In this study, a linear B cell epitope ₃₈₅LVGSGKGQI₃₉₃ (EP385) for DTMUV E protein were identified by truncated expressing the third domain of E protein (Domain III, D_III), which is recognized by monoclonal antibody BD10 prepared by the purified DTMUV YY5 strain as antigen, and then by scanning the peptide library which containing 15 overlapping segment of truncated and fused expressed D_III with BD10. Then, the immunogenicity of EP385 was verified by fusion expressed in E. coli and immunized mice to prepare a polyclonal antibody which was confirmed to be capable of recognizing viral E protein. The identification of B cell epitope for the E protein of DTMUV may lead to the preparation of a DTMUV peptide vaccine and the development of specific serological diagnostic methods.

Key words: duck Tembusu virus, monoclonal antibody, peptide library scanning, epitope

CLC Number:

S858.32

ZHANG Lin, YU Bin, NI Zheng, CHEN Liu, YUN Tao, YE Weicheng, HUA Jionggang, CUI Yanshun, ZHANG Cun. Identification of linear B cell epitope for E protein of duck Tembusu virus[J]. , 2017, 29(12): 2009-2014.

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Identification of linear B cell epitope for E protein of duck Tembusu virus

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