›› 2017, Vol. 29 ›› Issue (10): 1642-1647.DOI: 10.3969/j.issn.1004-1524.2017.10.07

• Animal Science • Previous Articles     Next Articles

Sequencing and B-cell epitopes prediction of Bordetella avium HagA protein

YUAN Peng, ZHU Ruiliang, CUI Chenchen, YANG Pingping*   

  1. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China
  • Received:2017-03-23 Online:2017-10-20 Published:2017-12-05

Abstract: This article is carried out to predict the secondary structure of Bordetella avium HagA and the dominant epitope of B cell antigen, in order to discuss the possibility of HagA protein in the production of monoclonal antibodies. In this study, hagA gene of B. avium was amplified, cloned and sequenced. The secondary structure and B cell epitopes of the HagA protein of B. avium were analyzed and predicted subsequently by using bioinformatics software. The results showed that the B. avium HagA protein was supposed to include 7 potential antigen epitopes and 6 potential glycosylated sites. The results provide a theoretical basis for the further study of immune mechanisms, monoclonal antibodies preparation and epitope vaccine design.

Key words: Bordetella avium, HagA, secondary structure, B-cell epitope prediction

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