Comparative analysis of BNK gene sequences in different chicken breeds

doi:10.3969/j.issn.1004-1524.2017.10.08

›› 2017, Vol. 29 ›› Issue (10): 1648-1653.DOI: 10.3969/j.issn.1004-1524.2017.10.08

• Animal Science • Previous Articles Next Articles

Comparative analysis of BNK gene sequences in different chicken breeds

CHEN Ying, ZHENG Shenghan, KONG Linglin, ZHU Pengfei, WU Yun, GUO Qixin, CHEN Guohong, CHANG Guobin^*

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2017-01-19 Online:2017-10-20 Published:2017-12-05

Abstract

Abstract: The BNK (C-type lectin-like receptor 2) gene is an inhibitory receptor expressed on natural killer cells, which is closely related to the innate immune function of poultry. In this study, according to NCBI 5.0 Gallus gallus, we used MEGA6 software to analyze BNK gene in 24 types of chickens. We found several SNPs, Indels and amino acids on exons and introns. In addition, we performed the clustering in 24 chickens via MEGA6. The results showed that most of the SNPs sites occurred on Exon3, Exon5 and Exon6, and most of them were missense mutations. The SNPs sites mostly occurred on Intron1, Intron2 and Intron4. And then the Indels mostly occurred on Intron2, Intron4 and Exon5. There was no significant difference between domestic and foreign chicken breeds, and most of the Indels sequences were basically the same. By cluster analysis the chickens were divided into three groups, Langshan chicken, Ross chicken, Recessive white chicken, Chahua chicken and Rugao yellow chicken as a class; Xueshan chicken, Anka chicken, Dou chicken and Xiaoshan chicken as a class; Gushi chicken as a class. According to clustering analysis, BNK gene of Gushi chicken was a single origin. In conclusion, BNK gene has abundant genetic diversity in Chinese chicken breeds and there was close relation in innate immunity with different chicken breeds, which provide reference for immune genetics and disease resistance breeding of poultry.

Key words: chicken, BNK gene, disease resistance, SNPs, Indels

CLC Number:

S831.2

CHEN Ying, ZHENG Shenghan, KONG Linglin, ZHU Pengfei, WU Yun, GUO Qixin, CHEN Guohong, CHANG Guobin. Comparative analysis of BNK gene sequences in different chicken breeds[J]. , 2017, 29(10): 1648-1653.

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Comparative analysis of BNK gene sequences in different chicken breeds

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