Overexpression of human Niemann-Pick C1-Like 1 in livers accelerates lipid peroxidation

doi:10.3969/j.issn.1004-1524.2017.10.09

›› 2017, Vol. 29 ›› Issue (10): 1654-1660.DOI: 10.3969/j.issn.1004-1524.2017.10.09

• Animal Science • Previous Articles Next Articles

Overexpression of human Niemann-Pick C1-Like 1 in livers accelerates lipid peroxidation

XU Chongli¹, XU Chongbo², GONG Yuchen³, OUYANG Hongsheng^{4, *}

1. College of Biology and Food Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China;
2. North Guangdong Collaborative Innovation and Development Center for Swine Farming and Disease Control, Yingdong College of Life Sciences, Shaoguan University, Shaoguan 512005, China;
3. College of Animal Science, Jilin Agricultural University, Changchun 130118, China;
4. College of Veterinary Medicine, Jilin University, Changchun 130062, China

Received:2016-10-12 Online:2017-10-20 Published:2017-12-05

Abstract

Abstract: The transgenic Bama miniature pigs in which human Niemann-Pick C1-Like 1(hNPC1L1) was overexpressed in the livers were constructed by using somatic cell nuclear transfer technique with the minipig fetal fibroblast cells as the nuclear donor cells. The hNPC1L1 was specifically expressed in transgenic liver tissues as revealed by PCR and reverse transcriptase (RT)-PCR analysis. A preliminary study on transgenic Bama miniature pigs was carried out, and the marker malondialdehyde (MDA) of lipid peroxidation was detected. The result showed that the level of MDA in transgenic pigs [(7.51±0.09) nmol·mL^-1] was significantly higher than that in non-transgenic pigs [(3.56±0.16) nmol·mL^-1] (P<0.001), but SOD level in transgenic pigs [(131±4.61)U·mg^-1] was significantly lower than that in non-transgenic pigs [(229.2±4.39)U·mg^-1] (P<0.001). All of these suggested that severe lipid peroxidation occurred in the liver and the oxygen free radical scavenging and antioxidant capacity were significantly reduced. This study revealed that overexpression of NPC1L1 in the liver led to severe lipid peroxidation, which indicated that NPC1L1 played an important role in lipid metabolism disorders and liver diseases and could be a new target for liver disease treatment.

Key words: human Niemann-Pick C1-Like 1, overexpression, somatic cell nuclear transfer, lipid peroxidation

CLC Number:

S828

XU Chongli, XU Chongbo, GONG Yuchen, OUYANG Hongsheng. Overexpression of human Niemann-Pick C1-Like 1 in livers accelerates lipid peroxidation[J]. , 2017, 29(10): 1654-1660.

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Overexpression of human Niemann-Pick C1-Like 1 in livers accelerates lipid peroxidation

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