Cloning, sequence analysis and tissue expression of MUSTN1 gene in sheep (Ovis aires)

doi:10.3969/j.issn.1004-1524.2017.10.10

›› 2017, Vol. 29 ›› Issue (10): 1661-1668.DOI: 10.3969/j.issn.1004-1524.2017.10.10

• Animal Science • Previous Articles Next Articles

Cloning, sequence analysis and tissue expression of MUSTN1 gene in sheep (Ovis aires)

GAO Jianfeng^{1, 2}, LU Zengkui^{1, 2}, MA Youji^{1, 2, *}, LI Taotao1^{1, 2}, ZHAO Xingxu³

1.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2.Gansu Engineering Laboratory of Sheep Breeding Biotechnology, Minqin 733300, China;
3.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received:2017-04-01 Online:2017-10-20 Published:2017-12-05

Abstract

Abstract: In order to study MUSTN1 gene characteristics of sheep (Ovis aires) and its expression at different developmental stages in longissimus dorsi and biceps femoris, fifteen sheep longissimus dorsi and biceps femoris tissues were collected at 0, 2, 6, 12 and 24 month old stages (three replicates for each). With application of molecular biology and other methods, the bioinformatics and tissue expression of the coding region of MUSTN1 gene were cloned and analyzed. The results showed that the CDS region of the MUSTN1 gene was 248 bp, encoded with 82 amino acid, molecular weight of 8.89 ku, its theoretical isoelectric point is 9.93, the GC content is greater than AT content, 5 phosphorylation sites, 5 N-glycosylation site, 1 O-glycosylation site, 0 transmembrane structure, hydrophobic protein and the secondary structure of MUSTN1 protein is dominated by random coil; the highest homology was 99.2% and 100% in the nucleotide and the amino acid sequence of goat (Capra hircus), followed by Bos taurus(97.2% and 100%, respectively). MUSTN1 gene was expressed in the longissimus dorsi and biceps femoris at different developmental stages (0, 2, 6, 12 and 24 month old). The expression of MUSTN1 in the longissimus dorsi was the highest at 24 month-old, which was significantly higher than those at other month old (P<0.01). The expression of MUSTN1 was the lowest at 0 month and significantly lower than that in 2 and 12 month old (P<0.01), significantly lower than that in 6 month old (P<0.05). The expression of MUSTN1 in 6 month old sheep biceps femoris was the highest and significantly higher than other month old (P<0.01). The expression level of 24 month old was significantly higher than that in 12 month old (P<0.01) and 0 month old (P<0.05). The conclusion was that the coding sequence of the MUSTN1 gene of sheep was conserved among species and expressed in the sheep of the dorsal long muscle and biceps femoris muscle at different development stages. The expression level of MUSTN1 gene may be related to the growth of skeletal muscle.

Key words: MUSTN1, sheep (Ovis aries), cloning, tissue expression, sequence analysis

CLC Number:

S826

GAO Jianfeng, LU Zengkui, MA Youji, LI Taotao1, ZHAO Xingxu. Cloning, sequence analysis and tissue expression of MUSTN1 gene in sheep (Ovis aires)[J]. , 2017, 29(10): 1661-1668.

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Cloning, sequence analysis and tissue expression of MUSTN1 gene in sheep (Ovis aires)

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