Prevalence and multilocus gene sequence analysis of Giardia duodenalis in captive non-human primates in parts of Southwest China

doi:10.3969/j.issn.1004-1524.2018.03.02

›› 2018, Vol. 30 ›› Issue (3): 366-370.DOI: 10.3969/j.issn.1004-1524.2018.03.02

• Animal Science • Previous Articles Next Articles

Prevalence and multilocus gene sequence analysis of Giardia duodenalis in captive non-human primates in parts of Southwest China

TIAN Yinan¹, WEI Bin¹, LI Ping², HUANG Xiangming³, LI Wei¹, TU Rui¹, DAN Jiaming¹, YUAN Li¹, YANG Tingyu¹, PENG Guangneng¹, ZHONG Zhijun^1,*

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Agricultural and Forestry Bureau of Qionglai, Qionglai 611530, China;
3. Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China

Received:2017-07-31 Online:2018-03-20 Published:2018-03-21

Abstract

Abstract: To understand the prevalence and molecular characteristics of Giardia duodenalis in captive non-human primates (NHPs) in parts of Southwest China, a total of 207 fresh fecal samples from rhesus macaques, northern white-cheeked gibbons, golden snub-nosed monkeys and crab-eating macaques were obtained from zoos, farms and laboratory in Southwest China. The oocysts in samples were collected by the Sheather's sucrose flotation technique, and their DNA was extracted. These DNA samples were used to amplify β-giardin (bg), tpi and gdh genes by nest PCR for detecting Giardia. The results showed that 7.73% (16/207) of samples were positive for G. duodenalis infection, and 16 positive samples from rhesus macaques, northern white-cheeked gibbons and crab-eating macaques were identified as assemblage B. Northern white-cheeked gibbons had the highest infection rate (38.89%), and the difference in infection rates among the species was significant (P<0.01). All the positive samples were successfully amplified based on bg, tpi and gdh genes, multilocus genotyping showed the high genetic diversity of bg and tpi. Based on a phylogenetic analysis, potentially zoonotic genotypes of Giardia duodenalis were identified in NHPs in southwest China.

Key words: non-human primates, Giardia duodenalis, bg, tpi, gdh, infection rate

CLC Number:

S855.9⁺9

TIAN Yinan, WEI Bin, LI Ping, HUANG Xiangming, LI Wei, TU Rui, DAN Jiaming, YUAN Li, YANG Tingyu, PENG Guangneng, ZHONG Zhijun. Prevalence and multilocus gene sequence analysis of Giardia duodenalis in captive non-human primates in parts of Southwest China[J]. , 2018, 30(3): 366-370.

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Prevalence and multilocus gene sequence analysis of Giardia duodenalis in captive non-human primates in parts of Southwest China

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