Genetic diversity in hulless barley by retrotransposons-based markers and simple sequence repeat (SSR) markers

doi:10.3969/j.issn.1004-1524.2018.03.01

›› 2018, Vol. 30 ›› Issue (3): 357-365.DOI: 10.3969/j.issn.1004-1524.2018.03.01

• Crop Science • Previous Articles Next Articles

Genetic diversity in hulless barley by retrotransposons-based markers and simple sequence repeat (SSR) markers

WANG Guorong¹, HUA Wei², CHEN Gonghai³, LONG Zhoukai¹, LI Bo¹, ZHANG Wenying¹, XU Yanhao^1,*

1. Hubei Collaborative Innovation Center for Grain Industry / School of Agriculture, Yangtze University, Jingzhou 434025, China;
2. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, National Barley Improvement Center, Hangzhou 310021, China;
3. Jingzhou Academy of Agricultural Sciences, Jingzhou 434000, China

Received:2017-11-09 Online:2018-03-20 Published:2018-03-21

Abstract

Abstract: To evaluate the genetic structure of hulless barley, retrotransposons-based markers (retrotransposon-microsatellite amplified polymorphism, REMAP; inter-retrotransposon amplified polymorphism, IRAP) and simple sequence repeat (SSR) markers were used to obtain the genetic data of 63 hulless barley materials. The IRAP, REMAP and SSR markers detected 315, 143 and 38 alleles, ranged from 9-58, 7-25, and 2-4, with an average of 24.23, 14.30, 2.38 alleles per marker, respectively. The contribution rate of single retrotransposons-based marker polymorphic site was higher than that of SSR marker. As revealed by retrotransposons-based markers, the genetic similarity (GS) among these 63 accessions was from 0.452 to 0.937, with the average of 0.674. The accessions were divided into two subgroups at the GS level of 0.620, which contained 20 and 43 materials, respectively. As revealed by SSR markers, GS among these 63 accessions was from 0.351 to 0.973, with the average of 0.716. The accessions were divided into two subgroups at the GS level of 0.620, which contained 2 and 61 materials, respectively. The wild hulless barley accessions was clustered into a small category by the retrotransposons-based markers at the GS level of 0.740. However, it was unable to distinguish the wild hulless barley from the cultured by SSR markers in the present study. The population structure revealed by retrotransposons-based markers was consistent with the principal coordinate analysis and genetic similarity analysis. However, the population structure revealed by SSR markers showed difference. It was shown that most of the accessions were with single genetic components. The retrotransposons-based markers had advantage in the identification of the relationship of hulless barleys. And more genetic information could be explored by combining the retrotransposons-based markers and SSR markers.

Key words: molecular markers, hulless barley, genetic diversity, population structure analysis

CLC Number:

S512.3

WANG Guorong, HUA Wei, CHEN Gonghai, LONG Zhoukai, LI Bo, ZHANG Wenying, XU Yanhao. Genetic diversity in hulless barley by retrotransposons-based markers and simple sequence repeat (SSR) markers[J]. , 2018, 30(3): 357-365.

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Genetic diversity in hulless barley by retrotransposons-based markers and simple sequence repeat (SSR) markers

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