›› 2018, Vol. 30 ›› Issue (3): 406-412.DOI: 10.3969/j.issn.1004-1524.2018.03.08

• Horticultural Science • Previous Articles     Next Articles

Identification of Clover yellow vein virus infecting broad bean in China by deep sequencing and characterization of virus-derived small interfering RNAs

ZHANG Chenhua1,2, ZHENG Hongying2, YAN Dankan3, HAN Kelei2,4, PENG Jiejun2, LU Yuwen2, LIN Lin2, ZHANG Dongfang3, CHEN Jianping1,2,*, YAN Fei2,*   

  1. 1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Stake Key Laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    3. Institute of Plant Protection and Agricultural Product Quality and Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China;
    4. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2017-11-30 Online:2018-03-20 Published:2018-03-21

Abstract: The broad bean samples causing prominent mosaic on leaves were collected in Hefei City, Anhui Province in China, and viruses in samples were detected by deep sequencing. The results showed that Clover yellow vein virus(ClYVV) existed in the broad bean samples and the complete genome ClYVV-HF was obtained by direct sequencing of the amplified genomic region, subsequently. The ClYVV genome of China consists of 9 585 nucleotides excluding the poly(A) tail, the full nucleotide sequence of ClYVV-HF shared a nucleotide identity of 95.45% and 95.10% with that of isolates from Japan(AB011819, NC_003536) and an isolate from Korea (KF975894) reported previously. Characterization of ClYVV-derived small interfering RNAs was further analyzed. The prevalent vsiRNAs size was 21-22 nt, and the 5'-terminal base of vsiRNAs was biased towards A/U. The ClYVV-derived small interfering RNAs were most derived from its genomic-strand RNAs, among the 7 hot spots across the genome, 5 hot spots were predominated by 21 nt vsiRNAs. Biological experiments showed that ClYVV could be used to frictionally inoculate many leguminous crops through juice. This is the first report about the full nucleotide sequence of ClYVV isolate from China.

Key words: broad bean, ClYVV, siRNA

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