Cloning and expression analysis of phytoene synthase gene from peach fruit

doi:10.3969/j.issn.1004-1524.2018.03.07

›› 2018, Vol. 30 ›› Issue (3): 399-405.DOI: 10.3969/j.issn.1004-1524.2018.03.07

• Horticultural Science • Previous Articles Next Articles

Cloning and expression analysis of phytoene synthase gene from peach fruit

LIANG Minhua¹, YANG Zhenfeng², SU Xinguo^1,*, SONG Chunbo³

1.School of Food Science, Guangdong Food and Drug Vocational College, Guangzhou 510520, China;
2.College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China;
3. College of Horticulture, South China Agricultural University, Guangzhou 510640, China

Received:2017-07-03 Online:2018-03-20 Published:2018-03-21

Abstract

Abstract: Full-length cDNAs of phytoene synthase (PSY) was isolated from peach fruits using gene cloning method. PpPSY was 1 532 bp in full length and encoded a predicted protein of 208 amino acids (ORF length 627 bp). Phylogenetic analysis indicated that PpPSY was similar to PSY from Prunus mume. Deduced amino acid sequence demonstrated that PpPSY protein contained substrate binding pocket, substrate-Mg²⁺ binding site, active site lid residues, catalytic residues and aspartate-rich region, which belonged to the isoprenoid biosynthesis enzymes Class 1 superfamily. The quantitative real-time PCR showed that expression level of PpPSY gene in developmental tissues of yellow flesh variety Jinli was higher than that of white flesh variety Hujing, and the expression of PpPSY gene in flower bud, hardcore fruit was significantly higher than that in flower, sprout and softcore fruit. For the cloning and expression analysis of PpPSY from peach fruits was reported, it lays the foundation for further research of carotenoid biosynthetic pathway.

Key words: peach fruits, PSY, gene cloning, expression analysis

CLC Number:

S662.1

LIANG Minhua, YANG Zhenfeng, SU Xinguo, SONG Chunbo. Cloning and expression analysis of phytoene synthase gene from peach fruit[J]. , 2018, 30(3): 399-405.

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Cloning and expression analysis of phytoene synthase gene from peach fruit

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