Study on phenotypic changes and expression of CsMADSs in over-expressing Arabidopsis plants

doi:10.3969/j.issn.1004-1524.2018.10.09

›› 2018, Vol. 30 ›› Issue (10): 1671-1679.DOI: 10.3969/j.issn.1004-1524.2018.10.09

• Horticultural Science • Previous Articles Next Articles

Study on phenotypic changes and expression of CsMADSs in over-expressing Arabidopsis plants

AN Yulan, ZHAI Keqing, YANG Feng, LEI Yue, HU Keling, GAN Defang^*, WANG Chenggang

Anhui Engineering Laborotary for Hoticultural Grop Breeding, College of Horticulture, Anhui Agricutural University, Hefei 230036, China

Received:2017-11-27 Online:2018-10-25 Published:2018-11-02

Abstract

Abstract: To investigate the CsMADSs genes which have influence on the growth and development of Arabidopsis thaliana, the eukaryotic expression vectors of CsMADS08 and CsMADS21 genes were constructed and transformed into tobacco to study their subcellular localization. At the same time, the eukaryotic expression vectors were transformed into Arabidopsis through Agrobacterium, and the positive transgenic plants were obtained. Meanwhile, phenotypic changes were observed in transgenic plants, and the expression of CsMADS08 and CsMADS21 were detected by real-time fluorescence quantitative PCR. The results showed that CsMADS08 was positioned on cell membrane and nucleus, while CsMADS21 was positioned on the cell membrane. In addition, purple leaves, fewer branches, and lotus leaves were detected on the lateral branch in the CsMADS08 transgenic plants. On the other hand, there were much more lateral inflorescences and pods, and also earlier flowering and fruits in the CsMADS21 transgenic plants than those of the wild plants. The fluorescent quantitative PCR results showed that CsMADS08 gene was expressed in the main stem, while CsMADS21 gene was mainly expressed in the flowers. The results provided a reference basis for further studying the function of CsMADSs genes and breeding new varieties of cucumber.

Key words: CsMADSs, Arabidopsis, subcellular localization, phenotypic change, expression analysis

CLC Number:

S649

AN Yulan, ZHAI Keqing, YANG Feng, LEI Yue, HU Keling, GAN Defang, WANG Chenggang. Study on phenotypic changes and expression of CsMADSs in over-expressing Arabidopsis plants[J]. , 2018, 30(10): 1671-1679.

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Study on phenotypic changes and expression of CsMADSs in over-expressing Arabidopsis plants

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