Cloning and characterization of maize ZmGS5 in regulating seed development of Arabidopsis thaliana

doi:10.3969/j.issn.1004-1524.2019.04.01

›› 2019, Vol. 31 ›› Issue (4): 513-518.DOI: 10.3969/j.issn.1004-1524.2019.04.01

• Crop Science • Previous Articles Next Articles

Cloning and characterization of maize ZmGS5 in regulating seed development of Arabidopsis thaliana

HE Chunmei, WANG Juan, DONG Rui, LIU Chunxiao, LIU Qiang, GUAN Haiying, WANG Liming, XU Xiangbo^*, LIU Tieshan

Maize Research Institute, Shandong Academy of Agricultural Sciences, National Engineering Laboratory of Wheat and Maize, Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-Huai River Plain, Ministry of Agriculture and Rural Affairs, Jinan 250100, China

Received:2018-07-16 Online:2019-04-25 Published:2019-04-19

Abstract

Abstract: Seed development is closely related with yield. Genome comparative analysis had revealed that maize ZmGS5 was significantly related with kernel development. In this study, the maize ZmGS5 was cloned, the open reading frame of ZmGS5 was 1 491 bp in length and encoded a 496 aa serine carboxypeptidase. The over-expression construct of ZmGS5 was then transformed into Arabidopsis by floral-dipping method. Through observation of the phenotypic alterations of the transgenic lines, functions of ZmGS5 in regulating growth and seed development was preliminarily revealed. These results showed that the transgenic lines over-expressing ZmGS5 could increase rosette diameter, enlarged leaf size and improved biomass, number of siliques per plant, one-thousand-seed weight and seed size. These results demonstrated that ZmGS5 played important roles in regulating plant growth especially in the seed size.

Key words: maize, ZmGS5, genetic transformation, Arabidopsis, seed size

CLC Number:

S513

HE Chunmei, WANG Juan, DONG Rui, LIU Chunxiao, LIU Qiang, GUAN Haiying, WANG Liming, XU Xiangbo, LIU Tieshan. Cloning and characterization of maize ZmGS5 in regulating seed development of Arabidopsis thaliana[J]. , 2019, 31(4): 513-518.

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Cloning and characterization of maize ZmGS5 in regulating seed development of Arabidopsis thaliana

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