Response mechanism and stress resistance of maize inbred lines to high temperature stress at seedling stage

doi:10.3969/j.issn.1004-1524.2019.07.03

›› 2019, Vol. 31 ›› Issue (7): 1045-1056.DOI: 10.3969/j.issn.1004-1524.2019.07.03

• Crop Science • Previous Articles Next Articles

Response mechanism and stress resistance of maize inbred lines to high temperature stress at seedling stage

FANG Fang, HE Xuchen, ZHANG Zhihao, ZHANG Qin, GUAN Yajing, HU Jin, HU Weimin^*

Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2018-12-18 Online:2019-07-25 Published:2019-08-07

Abstract

Abstract: High temperature stress affects the growth of maize inbred lines during seedling period and field tidiness as well as seed yield, therefore, exploring the response mechanism of maize seedling stage to temperature stress is conducive to breeding and screening varieties with high resistance. The mechanism was studied through the determination of chlorophyll content, antioxidant enzyme activity and gene expression of two different phenotypes after high temperature stress treatment at 45 ℃. The results showed that chlorophyll a and chlorophyll b content, chlorophyll a/b value, SOD activity, POD activity, CAT activity, APX activity and GR activity in heat sensitive inbred lines decreased dramatically compared with heat resistant lines after high temperature challenging and recovery experiment ,while the proline content increased slightly. The expression of ZmP5CS and ZmProDH, two key enzyme genes of proline metabolic pathway, and heat shock protein genes ZmHSP70, ZmHSP90 under high temperature stress were analyzed by qRT-PCR. Our result showed that ZmP5CS, ZmHSP70 and ZmHSP90 were significantly induced by heat stress, and the induction of these genes in heat sensitive inbred lines were mild in comparison with heat resistant inbred lines, while the expression of ZmProDH was significantly higher. These results showed that the accumulation of proline, activity of antioxidant enzymes and expression of heat shock protein genes involved in maize seedlings response to high temperature stress.

Key words: maize, high temperature stress, heat resistance, chlorophyll content, antioxidant enzyme activity

CLC Number:

S513

FANG Fang, HE Xuchen, ZHANG Zhihao, ZHANG Qin, GUAN Yajing, HU Jin, HU Weimin. Response mechanism and stress resistance of maize inbred lines to high temperature stress at seedling stage[J]. , 2019, 31(7): 1045-1056.

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Response mechanism and stress resistance of maize inbred lines to high temperature stress at seedling stage

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