Synergetic inhibition against cotton Verticillium wilt disease of arbuscular mycorrhizal fungi cooperated with Bacillus vallismortis

doi:10.3969/j.issn.1004-1524.2018.06.17

›› 2018, Vol. 30 ›› Issue (6): 1008-1015.DOI: 10.3969/j.issn.1004-1524.2018.06.17

• Plant Protection • Previous Articles Next Articles

Synergetic inhibition against cotton Verticillium wilt disease of arbuscular mycorrhizal fungi cooperated with Bacillus vallismortis

ZHANG Guoyi¹, CHENG Lin¹, HUANG Liying¹, QIAN Qihui¹, GUAN Linlin¹, YE Wenling¹, LU Hongjuan¹, RAN Wei², ZHANG Ligan^{1, *}

1. School of Resources and Environment, Anhui Provincial Key Laboratory of Farmland Ecological Conservation and Pollution Prevention and Control, Anhui Agricultural University, Hefei 230001, China;
2. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China

Received:2017-06-05 Online:2018-06-20 Published:2018-07-02

Abstract

Abstract: In the present study, the cooperative effect of arbuscular mycorrhiza fungi (AMF) Glomus versiforme and Bacillus vallismortis HJ-5 against cotton Verticillium wilt disease was explored by greenhouse pot experiment, and the possible underlying mechanisms was revealed by high performance liquid chromatography (HPLC) and tablet test. As compared with control, AMF and HJ-5 showed synergistic inhibition against Verticillium wilt disease, as the disease index was reduced by 50.82%, root weight was increased by 125.00%, and the colonization of HJ-5 in rhizosphere was enhanced by 23.80%. HPLC analysis showed that HJ-5 could produce antifungal substances, i.e. iturin A and surfactin. Dual inoculation significantly increased the concentration of iturin A and surfactin in rhizosphere than single inoculation of HJ-5 by 13.38% and 11.27%, respectively. It was hypothesized that the main mechanism of synergetic inhibition was that AMF promoted cotton root growth, helped HJ-5 colonized in rhizosphere, and produce more antifungal lipopeptides.

Key words: plant disease, economic crop, iturin A, surfactin

CLC Number:

S435.621

ZHANG Guoyi, CHENG Lin, HUANG Liying, QIAN Qihui, GUAN Linlin, YE Wenling, LU Hongjuan, RAN Wei, ZHANG Ligan. Synergetic inhibition against cotton Verticillium wilt disease of arbuscular mycorrhizal fungi cooperated with Bacillus vallismortis[J]. , 2018, 30(6): 1008-1015.

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Synergetic inhibition against cotton Verticillium wilt disease of arbuscular mycorrhizal fungi cooperated with Bacillus vallismortis

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