Expression of classical swine fever virus E2 protein in outer membrane vesicles of Escherichia coli and evaluation of its immunogenicity

doi:10.3969/j.issn.1004-1524.2018.09.03

›› 2018, Vol. 30 ›› Issue (9): 1460-1466.DOI: 10.3969/j.issn.1004-1524.2018.09.03

• Animal Science • Previous Articles Next Articles

Expression of classical swine fever virus E2 protein in outer membrane vesicles of Escherichia coli and evaluation of its immunogenicity

HUANG Jie¹, GONG Yongping¹, WEN Jifeng¹, YANG Rui², REN Lu², YAN Qigui^{1, 2, *}

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China

Received:2017-12-29 Online:2018-09-25 Published:2018-10-15
Contact: 颜其贵,E-mail: yanqigui@126.com
Supported by:
教育部“长江学者和创新团队发展计划”创新团队项目(IRT0848)

Abstract

Abstract: In order to investigate the immune effect of E. coli outer membrane vesicles (OMVs) as presentation vector of classical swine fever virus E2 protein, the classical swine fever virus E2 gene was ligated to the C-terminus of pBAD18-Cm-ClyA after excision of the transmembrane region, and then transformed into E. coli DH5α. Inducted with 0.2% arabinose, the OMVs containing the E2 protein were collected and the rabbits were immunized. Western blotting showed that the OMVs could specifically bind to CSFV antibody. Indirect ELISA showed that the recombinant OMVs could induce high level of IgG antibody in rabbits. After immunization, rabbits could well protect the live CSFV challenge. All these results indicated that E. coli CSFV E2 protein had good immunogenicity, which laid the foundation for the research of OMVs presentation of classical swine fever virus E2 protein vaccine.

Key words: OMVs, classical swine fever virus E2 protein, immunogenicity

CLC Number:

S858.28

HUANG Jie, GONG Yongping, WEN Jifeng, YANG Rui, REN Lu, YAN Qigui. Expression of classical swine fever virus E2 protein in outer membrane vesicles of Escherichia coli and evaluation of its immunogenicity[J]. , 2018, 30(9): 1460-1466.

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Expression of classical swine fever virus E2 protein in outer membrane vesicles of Escherichia coli and evaluation of its immunogenicity

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