›› 2018, Vol. 30 ›› Issue (9): 1467-1475.DOI: 10.3969/j.issn.1004-1524.2018.09.04

• Animal Science • Previous Articles     Next Articles

Screening of strains for inactivated vaccine of Erysipelothrix rhusiopathiae

WU Qiongjuan, YANG Zhipeng, YAO Yanbin, LU Ping, WEI Jianzhong, SUN Pei, LI Yu*   

  1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:2018-01-10 Online:2018-09-25 Published:2018-10-15
  • Contact: 李郁,E-mail: liyouer@163.com
  • Supported by:
    国家星火计划重点项目(2014GA710002); 安徽省质量工程项目(2013sxzx008); 安徽省生猪产业体系基金(皖农科〔2016〕84号)

Abstract: In order to screen out appropriate Erysipelothrix rhusiopathiae vaccine strains, which had strong pathogenicity, antigenicity and excellent genetic stability, four strains named AEr9, AEr21, AEr31 and AEr32 were screened from 42 clinically isolated strains of Erysipelothrix rhusiopathiae by lethality test in mice. The half lethal dose (LD50) of these strains was determined by modified Karber’s method, the reactivity was determined by microplate agglutination test and agar diffusion test, the immunogenicity was determined by immunization test in mice, and the genetic stability of the 10th, 20th, 30th generation of the tested strains was evaluated by LD50, precipitation antibody titer test and inactivated total bacterial immune protection rate test in mice. The results showed that the LD50 of AEr9, AEr21, AEr31 and AEr32 were 845, 50.3, 35.5 and 10.1 cfu·mL-1, respectively. The strains AEr21 and AEr31 with high reactivity were further screened by microplate agglutination test and agar diffusion test. The immune protection test showed that the immune protection of AEr31 was the best. Pathogenicity tended to be weakened in the virulence of the two strains after 10 generations, and the pathogenicity tended to be stable on the 10th, 20th and 30th generation strains, and the level of antibody precipitation of AEr21 was more stable. The results showed that the strains AEr21 and AEr31 could be used as candidate strains for inactivated vaccine of Erysipelothrix rhusiopathiae with the characteristics of strong virulence, good antigenicity and genetic stability.

Key words: Erysipelothrix rhusiopathiae, vaccine strain, pathogenicity, antigenicity, stability

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