Immunohistochemical localization of interleukin-21 in cecal tonsil of 42 day old Chinese Yellow Feather quail

doi:10.3969/j.issn.1004-1524.2018.09.05

›› 2018, Vol. 30 ›› Issue (9): 1476-1481.DOI: 10.3969/j.issn.1004-1524.2018.09.05

• Animal Science • Previous Articles Next Articles

Immunohistochemical localization of interleukin-21 in cecal tonsil of 42 day old Chinese Yellow Feather quail

LI Qin, HE Min^*, ZHAO Piao, CHEN Xiaomei, LYU Yingying

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2018-01-19 Online:2018-09-25 Published:2018-10-15
Contact: 何敏,E-mail: hemin09@163.com

Abstract

Abstract: To investigate the mechanism of interleukin-21 in the immune regulation of the Chinese Yellow Feather quail body, the distribution characteristics of IL-21 were observed. The quail tonsils of 42 day old Chinese Yellow Feather quail were taken, immunohistochemical SABC method was used for histological localization of interleukin-21 in the tonsil, and the morphology and distribution of the IL-21positive cells were observed. The results showed that the IL-21 positive reaction cells were round or elliptic, and a few were fusiform, some were clumpy. The positive reaction cells were mainly distributed in the lamina propria of the underlying layer of the epithelium of cecal tonsil, and a few were around the intestinal gland. There was also a small amount of IL-21 positive cells around the germinal centers. The results confirmed that IL-21 was widely distributed in the cecal tonsil of quail. The distribution and reaction strength of IL-21 positive cells were related to the local immune cells and the immune state of the body.

Key words: interleukin-21, Chinese Yellow Feather quail, cecal tonsil, localization

CLC Number:

S839
S852.1

LI Qin, HE Min, ZHAO Piao, CHEN Xiaomei, LYU Yingying. Immunohistochemical localization of interleukin-21 in cecal tonsil of 42 day old Chinese Yellow Feather quail[J]. , 2018, 30(9): 1476-1481.

References

[1] PARRISH-NOVAK J, DILLON S R, NELSON A, et al. Interleukin-21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function[J]. Nature , 2000, 408(6808):57-63.
[2] YI J S, COX M A, ZAJAC A J. Interleukin-21: a multifunctional regulator of immunity to infections[J]. Microbes Infect , 2010, 12(14/15):1111-1119.
[3] 刘巍,李为民,HUBER S A. 白细胞介素-21的免疫调节机制[J].国际免疫学杂志,2010,33(4):265-268.
LIU W, LI W M, HUBER S A. The immune regulatory function of interleukin-21[J]. International Journal of Immunology , 2010, 33(4):265-268. (in Chinese with English abstract)
[4] 张峰, 张雪莉, 刘冬, 等.白介素21的生物学功能与炎症性肠病的研究进展[J]. 世界华人消化杂志,2008, 16(19):2131-2136.
ZHANG F, ZHANG X L, LIU D, et al. Advances in biological function of interleukin-21 and inflammatory bowel disease[J]. World Chinese Journal of Digestology , 2008, 16(19):2131-2136. (in Chinese with English abstract)
[5] 王燕, 李佳缘, 李中原, 等. IL-21的研究进展及其在寄生虫学上的应用[J]. 中国兽医科学,2012,42(4):431-434.
WANG Y, LI J Y, LI Z Y, et al. Advance in interleukin-21 and its application to parasitology[J]. Veterinary Science in China , 2012, 42(4):431-434. (in Chinese with English abstract)
[6] 魏丽丽, 易发平, 宋方洲. 白介素21及其对肿瘤的抑制作用[J]. 生命的化学,2004,24(6):502-504.
WEI L L, YI F P, SONG F Z. Interleukin-21 and its function of tumor suppression[J]. Chemistry of Life , 2004, 24(6):502-504. (in Chinese with English abstract)
[7] 王士杰,鹿玲. 白细胞介素21的基础与临床研究进展[J]. 安徽医药,2009,13(9):1016-1018.
WANG S J, LU L. Basic and clinical researches of Interleukin-21[J]. Anhui Medical and Pharmaceutical Journal , 2009, 13(9):1016-1018. (in Chinese with English abstract)
[8] 陈晚华,刘辉,王延春,等. 表达人IL-21的细胞因子诱导的杀伤细胞对裸鼠肝癌的治疗研究[J]. 第二军医大学学报,2012,33(10):1045-1050.
CHEN W H, LIU H, WANG Y C, et al. Effect of cytokine-induced killer cells expressing hIL-21 on hepatocellular carcinoma in nude mice[J]. Academic Journal of Second Military Medical University , 2012, 33(10):1045-1050. (in Chinese with English abstract)
[9] 刘艳,万岁桂. 滤泡辅助性T细胞与自身免疫性疾病的研究进展[J]. 首都医科大学学报,2014,35(4):507-510.
LIU Y, WAN S G. Progress in T follicular helper cells and their related immune disease studies[J]. Journal of Capital Medical University , 2014, 35(4):507-510. (in Chinese with English abstract)
[10] MUNETA Y, KIKUMA R, YOSHIHARA K, et al. Cloning, expression, and tissue distribution of bovine interleukin-21[J]. Veterinary Immunology and Immunopathology , 2003, 95(1/2):73-80.
[11] 岳根华,李广宏. 鹌鹑黄羽自别雌雄配套系的建立: 鹌鹑黄羽隐性伴性基因的发现与鉴定[M]. 北京:中国农业科技出版社,1994.
[12] 赵一霞,何敏,杨欢,等. 血管活性肠肽在中国黄羽鹌鹑法氏囊中的定位[J]. 畜牧兽医学报,2014,45(8):1355-1362.
ZHAO Y X, HE M, YANG H, et al. Immunohistochemical localization of vasoactive intestinal peptide in bursa of Fabricious of Chinese yellow feather quails[J]. Acta Veterinaria et Zootechnica Sinica , 2014, 45(8):1355-1362. (in Chinese with English abstract)
[13] YANG Y R, SHE R P, ZHENG S M, et al. Effect of probiotics on intestinal mucosal immunity and ultrastructure of cecal tonsils of chickens[J]. Archives of Animal Nutrition , 2005, 59(4):237-246.
[14] 董红艳,张英楠,杨树宝,等. 鸡肠道相关淋巴组织研究进展[J]. 动物医学进展,2013,34(6):141-144.
DONG H Y, ZHANG Y N, YANG S B, et al. Research progress of intestinal tract associated lymphoid tissue[J]. Progress in Veterinary Medicine , 2013, 34(6):141-144. (in Chinese with English abstract)
[15] 杨树宝,张英楠,高晶晶,等. 鸡盲肠扁桃体中B淋巴细胞的发育[J]. 中国兽医科学,2009,39(11):999-1002.
YANG S B, ZHANG Y N, GAO J J, et al. Development of B lymphocytes in caecal tonsil of chicken[J]. Chinese Veterinary Science , 2009, 39(11):999-1002. (in Chinese with English abstract)
[16] Gómez D M M, FONFRIA J, VARAS A, et al. Appearance and development of lymphoid cells in the chicken ( Gallus gallus ) caecal tonsil[J]. Anatomical Record , 1998, 250(2):182-189.
[17] 栾维民,杨树宝,张英楠,等. 鸡盲肠扁桃体中T淋巴细胞及其亚群的发育[J]. 中国兽医科学,2008,38(4):338-341.
LUAN W M, YANG S B, ZHANG Y N, et al. Development of T lymphocyte subpopulations in caecal tonsil of chicken[J]. Chinese Veterinary Science , 2008, 38(4):338-341. (in Chinese with English abstract)
[18] 张立世,姜敏,杨树宝,等. 鸡盲肠扁桃体组织结构早期发育的动态观察[J]. 长春师范学院学报(自然科学版),2011,30(2):72-74.
ZHANG L S, JIANG M, YANG S B, et al. Dynamic observation on the early development of histological structure of cecal tonsil in chickens[J]. Journal of Changchun Normal University ( Natural Science ), 2011, 30(2):72-74. (in Chinese with English abstract)
[19] GLICK B, HOLBROOK K A, OLAH I, et al. An electron and light microscope study of cecal tonsil: the basic unit of the cecal tonsil[J]. Developmental Comparative Immunology , 1981, 5(1):95-104.
[20] 陈露,周晏丞,曹立亭,等. E. tenella 感染早期雏鸡盲肠扁桃体ChIL-17A和ChIL-17F表达水平的相反变化[J]. 中国兽医学报,2016,36(12):2074-2080.
CHEN L, ZHOU Y C, CAO L T, et al. Opposite regulation of ChIL-17A and ChIL-17F expression in chicken cecal tonsil in early period of E. tenella infection[J]. Chinese Journal of Veterinary Science , 2016, 36(12):2074-2080. (in Chinese with English abstract)
[21] 胡春蓉,石统东. IL-21与慢性乙型病毒性肝炎的关系研究[J]. 免疫学杂志,2011,27(10):913-917.
HU C R, SHI T D. IL-21 and chronic hepatitis B virus infection[J]. Immunological Journal , 2011, 27(10):913-917. (in Chinese with English abstract)
[22] 石荣亚. 血清IL-21在各型慢性乙肝、肝硬化及肝衰竭中的作用及其临床意义[D]. 石家庄:河北医科大学,2015.
SHI R Y. The role and clinical significance of serum IL-21in patients with chronic hepatitis B, cirrhosis and liver failure[D]. Shijiazhuang: Hebei Medical University, 2015. (in Chinese with English abstract)
[23] HABIB T, NELSON A, KAUSHANSKY K. IL-21: an novel IL-2-family lymphokine that modulates B, T, and natural killer cell responses[J]. Journal of Allergy and Clinical Immunology , 2003, 112(6):1033-1045.
[24] 逯志强. IL-21在鸵鸟免疫器官发育中的变化规律研究[D]. 武汉:华中农业大学,2012.
LU Z Q. Study on the changing rules of IL-21 in the ostrich immune organs development[D]. Wuhan: Huazhong Agricultural University, 2012. (in Chinese with English abstract)
[25] 易本谊,朱红霞,江和. 白细胞介素-21及其与相关疾病的研究进展[J]. 细胞与分子免疫学杂志,2009,25(11):1065-1067.
YI B Y, ZHU H X, JIANG H. Interleukin 21 and its progress in related diseases[J]. Chinese Journal of Cellular and Molecular Immunology , 2009, 25(11):1065-1067.(in Chinese)
[26] 刘志伟. IL-21和肥大细胞在乌鸡免疫器官发育过程中的分布及其变化[D]. 武汉:华中农业大学,2012.
LIU Z W. The distribution and changing of IL-21 and mast cells in silkie immune organs development [D]. Wuhan: Huazhong Agricultural University, 2012. (in Chinese with English abstract)
[27] 吴申,娄方方,孙建超,等. IL-21单独及联合5-FU对人胃癌细胞迁移、凋亡的影响[J]. 山东医药,2017,57(41):42-44.
WU S, LOU F F, SUN J C, et al. The effect of IL-21 alone and combined with 5-FU on the migration and apoptosis of human gastric cancer cells[J]. Shandong Medical Journal , 2017, 57(41):42-44. (in Chinese)

Immunohistochemical localization of interleukin-21 in cecal tonsil of 42 day old Chinese Yellow Feather quail

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments

[1]	GUO Lina, ZHAO Huiting, REN Youshe, XU Bing, JIANG Yusuo. Construction of insect expression vector pIB/V5-His of AcerOr2 in Apis cerana cerana [J]. , 2020, 32(6): 994-999.
[2]	XU Qingsong, ZHAO Jia, WEI Yunmin, HAN Rongrong, LIU Lusheng, JIANG Caode, YU Yong-xiong. Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa [J]. , 2019, 31(1): 11-19.
[3]	TANG Xiao, DENG Mengsheng, ZOU Xue, Li Liqin, ZHU Yuanzhi, WANG Xiyao. Cloning and expression analysis of StDWF1 in Solanum tuberosum [J]. , 2018, 30(6): 909-917.
[4]	ZHANG Li, LIU Teng, MIAO Qiuhong, TANG Jingyu, ZHU Jie, DONG Dandan, CHEN Zongyan, WANG Guijun, LIU Guangqing. Expression and subcellular localization of bone marrow stromal antigen 2 gene of Capra hircus [J]. , 2018, 30(5): 702-706.
[5]	CAO Xing, ZHANG Xiusheng, HOU Dong, SUI Juanjuan, MU Hongmei, GAO Xiangbin, LYU Futang, GUO Shangjing, WANG Guiqing. Cloning and characterization of LlMBF1a in lily induced by gray mold pathogen [J]. , 2018, 30(12): 2024-2030.
[6]	AN Yulan, ZHAI Keqing, YANG Feng, LEI Yue, HU Keling, GAN Defang, WANG Chenggang. Study on phenotypic changes and expression of CsMADSs in over-expressing Arabidopsis plants [J]. , 2018, 30(10): 1671-1679.
[7]	CHEN Bin;LI Dongyue;WANG Xuming;ZHOU Jie;YAN Chengqi;CHEN Jianping;*. Subcellular localization of rice resistance genes to bacterial blight [J]. , 2014, 26(3): 0-693701.
[8]	YANG Di;ZHOU Yanru;XIE Li;SUN Liying;CHEN Jianping;*. Subcellular immunolocalization of P2 protein encoded by Wheat yellow mosaic virus RNA2， using polyclonal antibody raised against prokaryotic expressed P2 fusion protein [J]. , 2014, 26(3): 0-742747.
[9]	BIAN Jing;XIANG Rong;SUN Li\\|ying;;CHEN Jian\\|ping;. N\\|terminal domain of VPg encoded by Wheat yellow mosaic virus is involved in the interaction with the nucleolar protein Fibrillarin [J]. , 2014, 26(1): 0-94.
[10]	ZHANG Yu;CONG Jing-hua;SHEN Ming-xia;*;XU Shui-yan;LIU Long-shen. Design and implementation of the forest-fire monitor and localization system based on ArcEngine [J]. , 2010, 22(5): 678-682.
[11]	CHEN Liu;YUN Tao;*;LIU Guang-qing;NI Zheng;YU Bin;SONG Yi;. Expression and localization analysis of VP60 protein of Rabbit hemorrhagic disease virus in insect cells\\|baculovirus expression system [J]. , 2010, 22(2): 0-139.