MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae

doi:10.3969/j.issn.1004-1524.2018.06.16

›› 2018, Vol. 30 ›› Issue (6): 999-1007.DOI: 10.3969/j.issn.1004-1524.2018.06.16

• Plant Protection • Previous Articles Next Articles

MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae

FENG Xiaoxiao¹, LI Haijiao², LI Ling^{3, 4}, WANG Jiaoyu^{3, *}, LIN Fucheng¹, LU Jianping^{2, *}

1. State Key Laboratory of Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, China;
2. College of Life Science, Zhejiang University, Hangzhou 310058, China;
3. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
4. College of Agriculture and Food Science, Zhejiang A and F University, Hangzhou 311300, China

Received:2018-01-05 Online:2018-06-20 Published:2018-07-02

Abstract

Abstract: SOK1 is a Ste20 protein kinase of the germinal center kinase (GCK) that is activated by oxidant stress and chemical anoxia. It is unknown so far whether SOK1 play roles in regulation of the fungal pathogenicity. Herein, we functionally characterized the SOK1 homologue in the rice blast fungus Magnaporthe oryzae(MoSOK1). MoSOK1 was up-regulated during conidial germination and appressorial formation. Targeted gene replacement showed that MoSOK1 acts as a regulator to fungal development and virulence in M. oryzae. Δmosok1 mutants showed decreased vegetative growth and conidiation, delayed conidial germination and a significant reduction in virulence. MoSOK1 was also found related to the sexual reproduction of the fungus. These data implicate that SOK1 signaling is required for fungal development and pathogenicity in phytopathogenic fungi.

Key words: Magnaporthe oryzae, fungal pathogenicity, MoSOK1

CLC Number:

S435.111.4⁺1

FENG Xiaoxiao, LI Haijiao, LI Ling, WANG Jiaoyu, LIN Fucheng, LU Jianping. MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae[J]. , 2018, 30(6): 999-1007.

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MoSOK1, a putative germinal center kinase encoding gene, is required for fungal growth, conidiation and pathogenicity in Magnaporthe oryzae

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