Secretory proteomics of Magnaporthe oryzae

doi:10.3969/j.issn.1004-1524.2018.11.13

›› 2018, Vol. 30 ›› Issue (11): 1899-1906.DOI: 10.3969/j.issn.1004-1524.2018.11.13

• Plant Protection • Previous Articles Next Articles

Secretory proteomics of Magnaporthe oryzae

CUI Shujian¹, WANG Jiaoyu², JIANG Hua², LIANG Jiansheng^1,*

1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-03-22 Online:2018-11-25 Published:2018-12-21

Abstract

Abstract: Secreted proteins of Magnaporthe oryzae play an important role during infection process on rice. In this study, the proteins enriched from the supernatant of the M. oryzae(Guy11 strain) culture were digested after alkylation and reduction treatment. The digested peptides were separated and identified using liquid chromatograph mass spectrometer (LC-MS/MS). Bioinformatic predictions of intracellular localization and function of these identified proteins were carried out using the SignalP and Protcomp softwares. Thirty secreted proteins of Magnaporthe oryzae were identified, among which, 24 proteins contained the signal peptide at the N-terminal, 26 proteins were capable of secreting out of the cell, and 3 proteins were localized on the cell membrane. Functional prediction indicated that most proteins were enzymes involved in hydrolysis and energy metabolism. Identification and further investigation of these enzymes and proteins would contribute to reveal the molecular pathogenesis of Magnaporthe oryzae infection.

Key words: Magnaporthe oryzae, secretome, bioinformatics

CLC Number:

S432.4⁺4

CUI Shujian, WANG Jiaoyu, JIANG Hua, LIANG Jiansheng. Secretory proteomics of Magnaporthe oryzae[J]. , 2018, 30(11): 1899-1906.

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