SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak

doi:10.3969/j.issn.1004-1524.2019.08.08

›› 2019, Vol. 31 ›› Issue (8): 1280-1285.DOI: 10.3969/j.issn.1004-1524.2019.08.08

• Animal Science • Previous Articles Next Articles

SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak

PENG Shuai, CHEN Lang, ZHENG Tianyu, LU Huining, ZHANG Li, LIU Lixia^*

College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China

Received:2018-12-29 Online:2019-08-25 Published:2019-08-30

Abstract

Abstract: The SNP sites of the coding region of TLR2 gene in Datong yak was screened by DNA mixed pool amplification and direct sequencing, and the allele frequency of each SNP site was estimated, and the effect of mutation to TLR2 mRNA and protein structure was predicted by the bioinformatics software. The results showed that there were two SNP sites in the coding region of Datong yak TLR2 gene, G⁶⁷⁷A and G¹⁵⁸⁷A respectively, of which G⁶⁷⁷A was missense mutation, resulting in the transformation of cysteine (Cys) to tyrosine (Tyr). The results of bioinformatics analysis showed that both G⁶⁷⁷A and G¹⁵⁸⁷A sites reduced the stability of the secondary structure of the RNA, and the secondary and tertiary structure of the protein also changed.

Key words: Datong yak, TLR2 gene, SNP site, bioinformatics

CLC Number:

S823.8⁺5

PENG Shuai, CHEN Lang, ZHENG Tianyu, LU Huining, ZHANG Li, LIU Lixia. SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak[J]. , 2019, 31(8): 1280-1285.

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SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak

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